+ Translate
+ Most Popular
Spermatogenic and steroidogenic impairment after chromium treatment in rats
Prediction of enthalpy of formation based on refined crystal structures of multisite compounds; Part 1, Theories and examples
Intrastromal photorefractive keratectomy for myopia by Nd:YLF picosecond laser
Teachers' beliefs about the role of prior language knowledge in learning and how these influence teaching practices
Classification and genesis of submarine iron-manganese deposits
Insecta a Cl. G. N. Potanin in China et in Mongolia novissime lecta. XVII. Hemiptera-Heteroptera
On the occurrence of the splendid genus Machaerothrix Haupt in Tsushima, with some remarks on the genus (Hymenoptera, Pompilidae)
Enamel craze lines
Gynecologic examination of the child and the adolescent
Periosteal osteoclasts, skeletal development and ageing
Fibrodysplasia ossificans progressiva in a Nigerian: a case report
The chronically ill patient with anorexia nervosa development, phenomenology, and therapeutic considerations
Kummelonautilus taiwanum sp. nov. (Nautilida) from the early Miocene Shihmen Formation, Chungliao, Nantou, west-central Taiwan
Efficacy of Droncit against Dipylidium caninum in dogs
Pierids of the genus Euchloe Klots (Lepidoptera, Pieridae) from Siberia and the Far East.
C5A anaphylatoxin and its seven transmembrane-segment receptor
Failure to practice evidence-based medicine: why do physicians not treat patients with heart failure with angiotensin-converting enzyme inhibitors?
Empirical comparisons of proportional hazards regression, Poisson regression, and logistic regression in the analysis of occupational cohort data
Pollination of flowers of sweet and sour cherries
Isopodi terrestri raccolti nell' Estremo Oriente
Morphometric observations on Dipylidium caninum from dogs and cats in Austria
Chelation effectiveness of zinc proteinates demonstrated
Alopecia--a psychosomatic disease picture? I. Review of the literature
Monitoring equity in health and healthcare: a conceptual framework
Hemodynamic evaluation of the Biocor heterologous prosthesis

The usefulness of alkaline solutions for clearing the uterus and staining implantation sites in rats

The usefulness of alkaline solutions for clearing the uterus and staining implantation sites in rats

Jikken Dobutsu. Experimental Animals 37(3): 325-331

ISSN/ISBN: 0007-5124

PMID: 2458273

The alkaline solution method for detecting uterine implantation sites (metrial glands or spots after delivery), which indicate the position of the embryo, was compared with conventional methods using Turnbull blue (Salewski's technique) and benzyl benzoate (Orsini's technique) in rats. When rat uteri were immersed directly in 2% sodium hydroxide [NaOH], potassium hydroxide [KOH] solution or one of several other alkaline solutions for one hour, the uteri were cleared. Then the metrial glands could be observed without further staining, and the spots after delivery with further staining. After alkaline treatment, unstained metrial glands appeared as yellowish-white degenerated masses and stained spots after delivery appeared as yellowish-brown patches covered with yellowish-white lumps. These yellowish-brown or yellowish-white sites were probably stained by the iron in hemosiderin deposits in the placental scar or in a vestige of the metrial glands left over from pregnancy. Formalin-fixed uteri were not cleared by immersion in a high concentration of NaOH, KOH or other alkaline solutions, but the spots after delivery were stained by this treatment. Turnbull blue did not stain the metrial glands, which contain no iron. Benzyl benzoate cleared fixed or unfixed uteri and allowed observation of the metrial glands and spots after delivery although the entire process required a few days and the specimens became hard and shriveled. The best and most convenient solutions for clearing the rat uterus and for staining implantation sites was 2% NaOH or KOH.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 006789733

Download citation: RISBibTeXText

Related references

Comparison of the alkaline solution method and Salewskis method in the usefulness for detecting implantation sites. Teratology 46(6): 37B, 1992

Expression of the novel gene embryo implantation factor 2 (EMO2) in the mouse uterus at the implantation sites. Fertility and Sterility 91(5 Suppl): 2116-2122, 2009

Monoclonal non-specific suppressor factor beta One of the genes down-regulated at implantation sites compared to interimplantation sites in the mouse uterus. Biology of Reproduction 60(Suppl. 1): 119, 1999

Identification of monoclonal nonspecific suppressor factor beta (mNSFbeta) as one of the genes differentially expressed at implantation sites compared to interimplantation sites in the mouse uterus. Molecular Reproduction and Development 55(4): 351-363, 2000

Changes of the luminal surface of the rat uterus at blastocyst implantation. Scanning electron microscopy and ruthenium red staining. Zeitschrift für Anatomie und Entwicklungsgeschichte 144(3): 337-342, 1974

Clearing and staining small vertebrates for demonstrating ossification of the skeletal system using the KOH and glycerine clearing method. References 48(6): 605-608, 2019

Asymmetrical distribution of implantation sites in the rat uterus. Biology of Reproduction 11(5): 611-618, 1974

Expression of adrenomedullin (ADM) and its binding sites in the rat uterus: increased number of binding sites and ADM messenger ribonucleic acid in 20-day pregnant rats compared with nonpregnant rats. Endocrinology 138(6): 2508-2514, 1997

A study on the differentiation of the implantation sites in the pseudopregnant rat uterus. Korean Journal of Zoology 34(4): 479-490, 1991

Reaction Of The Mouse Uterus During Implantation And Deciduoma Formation As Demonstrated By Changes In The Distribution Of Alkaline Phosphatase. Journal of Reproduction and Fertility 8: 331-338, 1964

The position and spacing of implantation sites in the uterus of the rat during early pregnancy. Journal of Reproduction and Fertility 20(3): 407-412, 1969

Estrogen and progestogen receptor kinetics in rat uterus at the implantation sites. Human Reproduction 2(Suppl. 1): 86-87, 1987

Extravascular plasma protein in the vicinity of implantation sites in the pregnant rat uterus. Proceedings of the North Dakota Academy of Science 31(1): 22, 1977

The staining of Brunner's gland and other neutral mucins by carmine, hematoxylin and orcein in alkaline solutions. Stain Technology 50(6): 383-386, 1975

Granulated metrial gland cells at implantation sites of the pregnant mouse uterus. Anatomy and Embryology 160(2): 227-238, 1980