Thermal stability characteristics of cellulase enzymes produced by sporotrichum thermophile
Margaritis, A.; Creese, E.
Biotechnology Letters 3(9): 471-476
1981
ISSN/ISBN: 0141-5492 DOI: 10.1007/bf00147556
Accession: 006797894
The thermal stability characteristics of the cellulase enzymes present in culture filtrates of the thermophilic fungus S. thermophile were investigated at different temperatures and at different times of exposure. Maximum enzymic activities under assay conditions were found at 68.degree. C for the filter paper activity (FPA) and the Cx activity (carboxymethylcellulose), while the maxima for the C1 activity (cotton) and .beta.-glucosidase activity (cellobiose) were at 55 and 72.degree. C, respectively. Culture filtrates were exposed to a given constant temperature for varying lengths of time to a maximum of 48 h and then analyzed for residual enzymic activities under assay conditions. The exposure temperatures studied were 50, 60 and 65.degree. C. After 48 h exposure time at 50.degree. C the residual activities for the FPA, Cx and .beta.-glucosidase were 88, 98 and 93% of the original activities, respectively.