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Thermostability of photosystem ii electron transport in the membranes of thermophilic cyanobacteria synechococcus elongatus



Thermostability of photosystem ii electron transport in the membranes of thermophilic cyanobacteria synechococcus elongatus



Fiziologiya Rastenii 34(6): 1073-1078



The thermal stability of membrane fragments in the thermophilic cyanobacteria Synechococcus elongatus was investigated in the presence of artificial acceptors-ferricyanide (FC) and 2.6-dichlorophenolindophenol (DCIP). A substantial differnce was found in the thermostability level between FC and DCIP reduction with water as electron donor and DCIP reduction with the donor diphenylcarbazide (DPC) in conditions of inactivated oxygen-evolving system. The temperature of a 50% inactivation of photosystem-II (PS-II) electron transport in the presence of FC, DCIP, and DPC .fwdarw. DCIP was 45, 58, and 73.degree. respectively. The FC reduction was the most sensitive to benzyl alcohol (BA) which modified the hydrophobic zone of the membrane. The DCIP reduction with water as electron donor was inhibited by much larger BA concentrations, and the DPC .fwdarw. DCIP system showed the highest stability to BA. The results obtained indicate that the reduction of FC and DCIP proceeds at different sites of PS-II electron transport chain. The site of FC reduction, which is likely to be associated with the secondary quinone acceptor, has a lower thermal stability and is sensitive to a modification of the membrane with hydrophobic agents. The site has a lower thermostability in vitro than that in native state, which seems to be a consequence of changes in the specific composition of external medium during isolation of membranes.

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