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Tobacco mosaic virus inoculation of tobacco protoplasts in the presence of protoplast fusion agents

Tobacco mosaic virus inoculation of tobacco protoplasts in the presence of protoplast fusion agents

Zeitschrift fuer Naturforschung Section C Journal of Biosciences 35(11-12): 1057-1061

Two methods used to induce protoplast fusion, involving polyethylene glycol (PEG) and high Ca/high pH, respectively, were investigated in procedures for the inoculation of isolated tobacco protoplasts with TMV. Inoculation in the presence of fusion agents involves 2 stages: virus precipitation onto the protoplast surface and entrapment of virus particles between protoplasts in zones (putatively) of transient protoplast fusion.

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Accession: 006811530

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