Toxic pathology of the hepatocyte in histiotypic culture part 2 activity of an organic chlorine compound lindane effect of a metabolizable foreign substance and toxic effect
Roux, F.; Guillam, C.; Brescol-Liversac, J.
Annales d'Anatomie Pathologique 23(4-5): 253-286
ISSN/ISBN: 0003-3871 Accession: 006817274
Lindane, .gamma.-hexachlorocyclohexane, is still used as an agricultural insecticide. Tests were carried out using primary cultures of chicken embryo hepatocytes at the 11th day of incubation, for 40 h, using different concentrations of the substance. At 0.5-10 .mu.mol/l, acid phosphatase fell significantly. At 25-50 .mu.mol/l, there was a tendency for mitochondrial hypertrophy and the granular and smooth endoplasmic reticulum and the Golgi system hyperplasia. Growth was unchanged and acid phosphatase decreased. At 75-125 .mu.mol/l, these changes were accompanied by signs of cellular damage and there was marked autophagia. Growth was reduced and acid phosphatase greatly decreased. At 150-175 .mu.mol/l, the lesions were markedly polymorphous and some indicative of impending cytolysis, in particular spiral tearing of the hyaloplasm. Growth was markedly impaired, while there was a paradoxal increase in acid phosphatase. In increasing concentration, lindane first had the effect of a metabolizable foreign substance of the phenobarbital type, then a definite toxic action. Sensitivity of the individual hepatocytes differed.