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Trace elements in human head hair 1. flameless atomic absorption analysis of calcium in hair and its variation in content


Trace elements in human head hair 1. flameless atomic absorption analysis of calcium in hair and its variation in content



Eisei Kagaku 27(6): 381-387



The contents of trace elements in hairs vary with difference of position in the same head, age, sex, hair cosmetic treatment and environmental pollution. The rapid analytical determination of Ca in small hair segments (1 cm) using flameless atomic absorption spectrophotometry and the variation of Ca content in hairs with permanent wave treatment were described. Ca was determined with the recovery of 99% by ashing in Pt crucible and dissolving the residue in water by stirring with the glass covered magnetic spin bar. Loss of Ca was observed when the porcelain crucible or the glass flask was used as ashing ware and the tefron covered magnetic spin bar caused contamination of Ca. Analytical results agreed with those of the other investigators determined by neutron activation analysis or atomic absorption analysis. In hairs which had never been permanent-waved, Ca concentrations were 200-1000 ppm and showed a general tendency to increase slightly from the root to the distal end of the hair. The permanent-waved hairs showed higher Ca concentrations (2000-4500 ppm). Adsorption of Ca on treated hairs depended on pH, showing that after the hair previously treated with permanent wave reagents was soaked in city water (pH 6.8) Ca content increased and no significant increase was observed by soaking in acidic Ca solution (pH 3). Ca adsorbed on the treated hairs was removed by soaking in dilute HCl (1 .fwdarw. 20). Permanent wave treatment increased ion-exchangeable Ca in the hair.

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