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Trace enrichment and separation of cholesterol oxidation products by adsorption high performance liquid chromatography

Trace enrichment and separation of cholesterol oxidation products by adsorption high performance liquid chromatography

Journal of Chromatography 241(2): 381-389

ISSN/ISBN: 0021-9673

DOI: 10.1016/s0021-9673(00)81762-8

A method for the liquid chromatographic determination of some oxidation products of cholesterol is described. The oxygenated sterols were concentrated on a short pre-column of Nucleosil NO2 with hexane as the mobile phase. The separation was performed on a longer column with the same stationary phase but with a hexane-rpopanol or a hexane-butanol gradient. Cholest-5-ene-3.beta.,20.alpha.-diol, cholest-5-ene-3.beta.,25-diol cholest-5-ene-3.beta.,7.alpha.-diol, cholest-5-ene-3.beta.,7.beta.-diol, 3.beta.-hydroxycholest-5-ene-7-one and cholest-5-en-3.beta.-ol were detected at levels as low as 10-8 mol .cntdot. 1-1. The method was also used for the determination of oxidation products of cholesterol in butter and in heated butter.

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Accession: 006819994

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