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Transcriptional initiation and 5' termini of yeast mitochondrial RNA

Transcriptional initiation and 5' termini of yeast mitochondrial RNA

Journal of Biological Chemistry 256(10): 5226-5232

We have used vaccinia virus guanylyltransferase to label polyphosphate-terminated yeast mitochondrial RNAs in vitro with [alpha-32P]GTP. Hybridization of RNA labeled in vitro indicates the presence of multiple transcriptional initiation sites in both grande and petite mitochondrial genomes. Agarose/urea gel electrophoresis of capped RNA suggests the existence of a precursor to the small (14 S) rRNA. In contrast, direct examination of the large (21 S) rRNA by partial ribonuclease T1 digestion reveals a complete lack of processing of the 5' end of the primary transcript of this RNA.

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Accession: 006823625

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PMID: 6262322

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