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Transfer of glucose in the biosynthesis of thyroid glyco proteins 1. inhibition of glucose transfer to oligo saccharide lipids by gdp mannose

Transfer of glucose in the biosynthesis of thyroid glyco proteins 1. inhibition of glucose transfer to oligo saccharide lipids by gdp mannose

Biochimica et Biophysica Acta 674(1): 48-57

Porcine thyroid rough microsomes catalyzed the synthesis of glucose-containing oligosaccharide lipids which were compared to those extracted from labeled thyroid cells and were largely similar. Glucose transfer to these oligosaccharide lipids in the microsomal system was markedly depressed by an addition of GDP-mannose. This sugar nucleotide, already at 1 .mu.M, blocked dolichol-P-glucose synthesis, thus restraining further glucosylation of oligosaccharide lipids. Using this concentration of radioactive GDP-mannose in the incubation medium led to the detection of 3 glucose containing mannose-labeled oligosaccharide lipids. Double labeling experiments suggested a precursor-product relationship between them. Previously labeled oligosaccharide lipids, either containing or not containing glucose, were compared in their efficiency to act as donors of their oligosaccharide chain to an exogenous synthetic Asn-X-Thr containing peptide. The presence of glucose did not significantly influence the transfer. Free glucose was released during the reaction when using the glucose-labeled oligosaccharide lipid.

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Accession: 006824751

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PMID: 6165397

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