Section 7
Chapter 6,827

Transformation of human cells by DNA transfection

Sutherland, B.M.; Bennett, P.V.

Cancer Research 44(7): 2769-2772


ISSN/ISBN: 0008-5472
PMID: 6586290
Accession: 006826160

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Normal human cells can be transformed to anchorage-independent growth by transfection with DNA from MOLT-4 lymphoblasts, derived from a patient with acute lymphocytic leukemia. Cells were treated with polyethylene glycol 6000, and DNA was administered as a calcium phosphate coprecipitate . The transforming activity of MOLT DNA was inactivated by treatment with DNase or by the restriction enzymes Bgll or BamHl but not by EcoRV . DNA from transfected colonies can transform human recipient cells to anchorage-independent growth; the transforming ability and anchorage independence are maintained stably for at least 30 generations.

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