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Transport of 3-phosphoglyceric acid, phosphoenolpyruvate, and inorganic phosphate in maize mesophyll chloroplasts,, and the effect of 3-phosphoglyceric acid on malate and phosphoenolpyruvate production



Transport of 3-phosphoglyceric acid, phosphoenolpyruvate, and inorganic phosphate in maize mesophyll chloroplasts,, and the effect of 3-phosphoglyceric acid on malate and phosphoenolpyruvate production



Archives of Biochemistry & Biophysics 211(2): 743-749



Maize mesophyll chloroplasts loaded with radioactively labeled 3-phosphoglycerate or phosphoenolpyruvate exchange these compounds for externally provided Pi, 3-phosphoglycerate, phosphoenolpyruvate and dihydroxyacetone phosphate. These exchanges are inhibited by pyridoxal phosphate. 3-Phosphoglycerate uptake, which leads to accumulation of this substance in the stroma, is competitively inhibited by Pi and phosphoenolpyruvate. These results are consistent with the transport of 3-phosphoglycerate, phosphoenolpyruvate, Pi and dihydroxyacetone phosphate being mediated by a common carrier (the phosphate translocator). The activation energy of 3-phosphoglycerate uptake as determined from its temperature dependence is 19.5 kcal (4.degree.-15.degree. C). In isolated chloroplasts malate and phosphoenolpyruvate production from oxalacetate and pyruvate, respectively, is inhibited by 3-phosphoglycerate, the extent of inhibition being dependent on the relative concentrations of Pi and 3-phosphoglycerate. 3-Phosphoglycerate from bundle-sheath cells may serve as a feedback regulator of C4 mesophyll cell photosynthesis.

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Accession: 006831289

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PMID: 7305395


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