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Trehalose absorption related to trehalase in developing ovaries of the silkworm bombyx mori






Journal of Comparative Physiology B Biochemical Systemic & Environmental Physiology 131(4): 333-340

Trehalose absorption related to trehalase in developing ovaries of the silkworm bombyx mori

Trehalose absorption was examined in developing ovaries of B. mori Trehalose and glucose absorption followed saturation kinetics giving an apparent Km value of 8.4 mM and a Vmax of 12.5 .mu.moles/30 min/g ovaries for trehalose absorption, and an apparent Km value of 26.4 mM and a Vmax of 36.6 .mu.moles/30 min/g ovaries for glucose uptake. Trehalose absorption was clearly inhibited by addition of NaCN or NaN3 to the incubation medium. Cellobiose, maltose, sucrose and turanose were taken up by ovaries at much lower rates than trehalose. Among the disaccharidases which hydrolyse these sugars, trehalase activity was highest. The correlation between trehalase activity and trehalose absorption rate was demonstrated by a reduction of trehalase activity accompanied by reduced absorption rates after extirpation of the suboesophageal ganglion (SG). During trehalase absorption, glucose was released into the incubation medium, but after SG removal, no liberation of glucose was observed. No accumulation of 14C-trehalose, added to the medium was observed in the cells and almost all radioactivity was recovered as glucose and glycogen in the ovaries. These results suggest that in developing silkworm ovaries, trehalose is absorbed by a specific carrier-mediated and energy-dependent system, in which the hydrolysis by trehalase is an obligatory step.


Accession: 006839075



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