+ Translate

Trehalose absorption related to trehalase in developing ovaries of the silkworm bombyx mori

, : Trehalose absorption related to trehalase in developing ovaries of the silkworm bombyx mori. Journal of Comparative Physiology B Biochemical Systemic & Environmental Physiology 131(4): 333-340

Trehalose absorption was examined in developing ovaries of B. mori Trehalose and glucose absorption followed saturation kinetics giving an apparent Km value of 8.4 mM and a Vmax of 12.5 .mu.moles/30 min/g ovaries for trehalose absorption, and an apparent Km value of 26.4 mM and a Vmax of 36.6 .mu.moles/30 min/g ovaries for glucose uptake. Trehalose absorption was clearly inhibited by addition of NaCN or NaN3 to the incubation medium. Cellobiose, maltose, sucrose and turanose were taken up by ovaries at much lower rates than trehalose. Among the disaccharidases which hydrolyse these sugars, trehalase activity was highest. The correlation between trehalase activity and trehalose absorption rate was demonstrated by a reduction of trehalase activity accompanied by reduced absorption rates after extirpation of the suboesophageal ganglion (SG). During trehalase absorption, glucose was released into the incubation medium, but after SG removal, no liberation of glucose was observed. No accumulation of 14C-trehalose, added to the medium was observed in the cells and almost all radioactivity was recovered as glucose and glycogen in the ovaries. These results suggest that in developing silkworm ovaries, trehalose is absorbed by a specific carrier-mediated and energy-dependent system, in which the hydrolysis by trehalase is an obligatory step.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006839075

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Szponar, L.; Traczyk, I.; Jarzebska, M.; Stachowska, E., 2003: Estimation of food additives intake - methods applied in the European Union. Guidelines for the estimation of food additive intake were prepared by the Codex Committee on Food Additives and Contaminants (CCFAC) in 1989. During the period from 1994 to 1995, three different directives have been adopted, with each including a...

Peter J. Weisberg; Susan G. Mortenson; Thomas E. Dilts, 2012: Gallery Forest or Herbaceous Wetland? The Need for Multi-Target Perspectives in Riparian Restoration Planning. Much riparian restoration focuses on establishment of gallery forests, with relatively limited effort to restore herbaceous wetlands as key components of riparian landscape mosaics. Multiple reasons for this include inherent cultural or esthetic p...

Vanegmond E.M.; Raju U.; Torres F.X.; Linden M.D.; Zarbo R.J., 1992: Correlation of c erbb 2 oncoprotein immunostaining with dna ploidy and nuclear grade in mammary ductal carcinomas. Laboratory Investigation 66(1): 19A

Eridani S.; Gorman P.; Sheer D.; Duncombe A.S.; Glass U.H.; Shivji M.K.K., 1987: Monosomy 7 and ph positive acute lymphoblastic leukemia cytogenetic and molecular aspects. A combination of monosomy 7 and translocation t(9;22) (q34;q11), rarely observed in acute lymphoblastic leukaemia (ALL), is here reported: a peculiarity of this case was that the "breakpoint cluster region" on chromosome 22 was not rearr...

Brasil, L., 1916: Deux captures interessantes pour le Calvados. Revue Francaise d'Ornithologie, 8 324-325

Hirsch, Hans, H., 2005: BK virus: Opportunity makes a pathogen. More than 70% of the general population worldwide has serological evidence of exposure to Polyomavirus hominis type 1, better known as BK virus ( BKV). BKV infection typically occurs during childhood, without specific symptoms, followed by a state...

Musicki, Biljana; Champion, Hunter, C.; Becker, Robyn, E.; Liu, Tongyun; Kramer, Melissa, F.; Burnett, Arthur, L., 2005: Erection capability is potentiated by long-term sildenafil treatment: Role of blood flow-induced endothelial nitric-oxide synthase phosphorylation. Despite demonstrated clinical efficacy of sildenafil for the temporary treatment of erectile dysfunction, the possibility that sildenafil used long-term durably augments erectile ability remains unclear. We investigated whether continuous long-ter...

Rodriguez R.; Skroback D., 1986: Seismic methods to predict the geometry and coal quality of coal seams. An economic in-house seismic method has been developed to map the structure of coal seams and determine the coal seam quality in Hazard, Southeastern Kentucky. High-resolution surface reflection techniques were used to investigate the geometry of...

Nakamura, Shin-Ichi; Hayashi, Hiroaki; Chino, Mitsuo, 2006: Detection of calmodulin and calmodulin-binding proteins in pure phloem sap of rice plants. Calcium-ligand blots using superscript 45Ca[superscript 2[+]] revealed that there were at least four calcium-binding proteins (17, 18.5, 48 and 52 kDa) in the phloem sap of rice plants. Furthermore, an anti-spinach calmodulin antibody cross-react...

Arambarri, Angelica, M.; Cabello, Marta, N., 1995: Menispora gamundiae sp. nov., a new Hyphomycete from Argentina (Buenos Aires province). Menispora gamundiae a new species of dematiaceous hyphomycete, found in litter floating on water in a Buenos Aires province stream in Argentina, is proposed.