EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Trehalose metabolism by Bacillus popilliae


, : Trehalose metabolism by Bacillus popilliae. Journal of Bacteriology 119(2): 484-493

Trehalose was found to be utilized more readily than glucose for the growth of Bacillus popilliae NRRL B-2309MC. The pathway of degradation of trehalose was elucidated and found to differ from that reported for other organisms. Trehalase and trehalose phosphorylase activities could not be detected. Rather, trehalose was found to undergo phosphoenolpyruvate (PEP)-dependent phosphorylation, and the resulting trehalose 6-phosphate was cleaved by a phosphotrehalase to equimolar amounts of glucose and glucose 6-phosphate. The phosphotrehalase was purified 34-fold and shown to have a pH optimum of 6.5 to 7.0 and a K(m) for trehalose 6-phosphate of 1.8 mM. A mutant missing the phosphotrehalase failed to grow on trehalose but grew normally on other sugars. The mutant accumulated [(14)C]trehalose as [(14)C]trehalose 6-phosphate. Phosphorylation of trehalose by dialyzed extracts was at least 25 times faster with PEP than with adenosine 5'-triphosphate, and the phosphorylation activity was associated primarily with the particulate fraction. These data and the results of studies of [(14)C]trehalose uptake suggest that trehalose is transported into the cell as trehalose 6-phosphate by a PEP:sugar phosphotransferase system. Cell extracts of other strains of B. popilliae were also found to produce [(14)C]sugar phosphate from [(14)C]trehalose and to have phosphotrehalase activity.


Accession: 006839089

PMID: 4369400

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Bhumiratana A.; Costilow R.N., 1973: Trehalose metabolism in bacillus popilliae. Abstracts of the Annual Meeting of the American Society for Microbiology 73: 187

Steinkraus, K.H., 1957: Studies on the milky disease organisms. I. Parasitic growth and sporu-lation of Bacillus popilliae. II. Saprophytic growth of Bacillus popilliae. III. Variability among strains of Bacillus popilliae sporulating on artificial media. Journal of Bacteriology Baltimore, 74: 621-624

Bulla, L.Jr, 1977: Physiology of sporeforming bacteria associated with insects: enzymatic analysis of the aerobic and anaerobic metabolism of Bacillus popilliae, Bacillus lentimorbus, and Bacillus larvae. Spore Research 1976: ub 1977), 1 407-419

Kaya, H.; Klein, M.; Burlando, T., 1993: Impact of Bacillus popilliae, Rickettsiella popilliae and entomopathogenic nematodes on a population of the scarabaeid, Cyclocephala hirta. Larvae of the scarabaeid, Cyclocephala hirta, are major pests of turfgrass in California. A field test was conducted against third instars that included the following treatments: untreated control; chemical insecticide (bendiocarb): milky disease...

Hanula, JL., 1990: Epizootiological investigations of the microsporidium Ovavesicula popilliae and bacterium Bacillus popilliae in field populations of the Japanese beetle (Coleoptera: Scarabaeidae). Population densities of Popillia japonica and prevalence of the pathogens Ovavesicula popilliae and Bacillus popilliae Dutky are reported from 3 locations in Connecticut over a 3-year period. Populations decreased to very low levels (<1 larva/0...

Bulla L.A.Jr; S.J.lian G., 1972: Lipid metabolism during bacterial growth and sporulation phospho lipid pattern in bacillus thuringiensis and bacillus popilliae. Halvorson, Harlyn O , Richard Hanson And L Leon Campbell (Ed ) Spores V 5th International Spore Conference Fontana, Wisconsin, Oct 8-10, 1971 X+471p Illus American Society For Microbiology: Washington, D C , U S A 191-196

Luthy, P., 1975: Control of insect pests with bacteria, the entomophagous Bacillus species, Bacillus thuringiensis and Bacillus popilliae

Luthy, P., 1981: Bacteriological pest control: the entomopathogenic bacillus species: Bacillus thuringiensis and Bacillus popilliae

Bakhiet, N.; Stahly, D.P., 1985: Studies on transfection and transformation of protoplasts of Bacillus larvae, Bacillus subtilis, and Bacillus popilliae. Protoplasts of Bacillus larvae NRRL b-3555 and Bacillus subtilis RM125 (restrictionless, modificationless mutant) were transfected with DNA from the B. larvae bacteriophage PBL1c in the presence of polyethylene glycol. B. subtilis 168 and Bacillus...

S.J.lian, G.; Bulla, L.A.; Hanson, R.S., 1975: Physiology of sporeforming bacteria associated with insects: metabolism of Bacillus popilliae grown in third-instar Popillia japonica Newman larvae. The timing and relative participation of concurrent pathways of carbohydrate metabolism as well as the extent of terminal respiratory activity were determined by radiorespirometry with 14-C substrates and by enzyme assays for vegetative and sporul...