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Treponema saccharophilum new species a large pectinolytic spirochete from the bovine rumen






Applied & Environmental Microbiology 50(2): 212-219

Treponema saccharophilum new species a large pectinolytic spirochete from the bovine rumen

A large, obligately anaerobic spirochete (strain PB) was isolated from bovine rumen fluid by a procedure involving rifampin as a selective agent. The helical cells measured 0.6-0.7 .mu.m by 12-20 .mu.m and possessed .apprx. 16 periplasmic flagella inserted near each end of the protoplasmic cylinder. The periplasmic flagella were arranged in a bundle wound around the cell body. Strain PB utilized as fermentable substrates various plant polysaccharides (e.g., pectin, arabinogalactan, starch and inulin) and pentoses, hexoses, disaccharides and uronic acids. Glucose was fermented to acetate, formate and ethanol, whereas the fermentation of pectin or glucuronic acid yielded only acetate and formate as major end products. Determinations of radioactivity in end products and assays of enzymatic activities indicated that strain PB catabolized glucose via the Embden-Meyerhof pathway. Extracts of cells grown in pectin-containing media possessed relatively high levels of phospho-2-keto-3-deoxygluconate aldolase activity, an enzymatic activity typical of the Entner-Doudoroff pathway. The guanine-plus-cytosine content of the DNA of strain PB (54 mol%) was considerably higher than that of known host-associated anaerobic spirochetes. Strain PB represents a new Treponema sp. for which the name T. saccharophilum is proposed.


Accession: 006839639



Related references

Paster, B.J.; Canale-Parola, E., 1985: Treponema saccharophilum sp. nov., a large pectinolytic spirochete from the bovine rumen. A large, obligately anaerobic spirochete (strain PB) was isolated from bovine rumen fluid by a procedure involving rifampin as a selective agent. The helical cells measured 0.6 to 0.7 micron by 12 to 20 micron and possessed approximately 16 peripl...

Liu, J.; Wang, J-Kun.; Zhu, W.; Pu, Y-Yi.; Guan, L-Luo.; Liu, J-Xin., 2014: Monitoring the rumen pectinolytic bacteria Treponema saccharophilum using real-time PCR. Treponema saccharophilum is a pectinolytic bacterium isolated from the bovine rumen. The abundance of this bacterium has not been well determined, reflecting the lack of a reliable and accurate detection method. To develop a rapid method for monit...

Rosewarne, C.P.; Cheung, J.L.; Smith, W.J.M.; Evans, P.N.; Tomkins, N.W.; Denman, S.E.; Ó Cuív, Páraic.; Morrison, M., 2012: Draft genome sequence of Treponema sp. strain JC4, a novel spirochete isolated from the bovine rumen. Morphologically and biochemically diverse members of the Treponema genus are present in the gastrointestinal tract of ruminants, yet very little is understood about their functional importance to this microbiome. Here we describe the annotated dra...

Stanton T.B.; Canale Parola E., 1980: Treponema bryantii new species a rumen spirochete that interacts with cellulolytic bacteria. A saccharolytic spirochete that associated and interacted with cellulolytic bacteria was isolated from bovine rumen fluid. Isolation was accomplished by means of a procedure involving serial dilution of a sample of rumen fluid into a cellulose-con...

Tadese, T.K.omparens, K.; Benson, M.; Rust, S.; Yokoyama, M., 1990: Association of rumen bacteriophages with Treponema saccharophilum and Butyrivibrio fibrisolvens. Research report from the Michigan State University Agricultural Experiment Station East Lansing: (491) 206-210

Kasperowicz, A.; Míchalowski, T., 2002: Assessment of the fructanolytic activities in the rumen bacterium Treponema saccharophilum strain S. To characterize the fructose polymer degrading enzymes of rumen bacterium Treponema saccharophilum strain S. Conventional methods were used to examine bacterial growth and enzyme activities. Electrophoretic zymogram under native conditions, and th...

Kasperowicz, A.M.chalowski, T., 2002: Assessment of the fructanolytic activities in the rumen bacterium Treponema saccharophilum strain S. Aims: To characterize the fructose polymer degrading enzymes of rumen bacterium Treponema saccharophilum strain S. Methods and Results: Conventional methods were used to examine bacterial growth and enzyme activities. Electrophoretic zymogram unde...

Stanton, T.B., 1984: Glucose metabolism of Treponema bryantii, an anaerobic rumen spirochete. The pathway of glucose metabolism by Treponema bryantii, an obligately anaerobic spirochete isolated from bovine rumen contents, was studied. Washed cell suspensions of the spirochete consumed glucose and CO2 and produced equimolar amounts of acet...

Stanton, T.B.; Canale-Parola, E., 1980: Treponema bryantii sp. nov., a rumen spirochete that interacts with cellulolytic bacteria. A saccharolytic spirochete that associated and interacted with cellulolytic bacteria was isolated from bovine rumen fluid. Isolation was accomplished by means of a procedure involving serial dilution of a sample of rumen fluid into a cellulose-con...

Liu, J.; Pu, Y-Yi.; Xie, Q.; Wang, J-Kun.; Liu, J-Xin., 2015: Pectin induces an in vitro rumen microbial population shift attributed to the pectinolytic Treponema group. Pectin is a non-fiber carbohydrate (NFC) that exists in forages, but it is not clear how pectin exerts its effect on populations of either known microbial species or uncultured ruminal bacteria. PCR-denaturing gradient gel electrophoresis (PCR-DGG...