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Trh in the rat cerebellum 2. uptake by cerebellar slices

, : Trh in the rat cerebellum 2. uptake by cerebellar slices. Peptides (Orlando) 2(3): 283-288

The in vitro uptake of exogenous TRH by rat cerebellar slices was investigated. TRH was taken up via a process sharing many of the properties of a high affinity transport system, i.e., saturation kinetics; high affinity kinetic constants (Km1 = 1.06 .times. 10-5 M, Km2 = 5.6 .times. 10-6 M); temperature sensitivity (Q10 = 1.48); dependency on the Na concentration in the incubation medium; and tissue/medium ratios greater than 1. After 60 min of incubation at C in the presence of bacitracin, the tissue/medium ratio was 5:1. Under these conditions 70% of the total label was recovered as [3H-Pro]TRH. These findings demonstrated the existence of removal mechanisms for TRH in the cerebellum and gave further support to the hypothesis that TRH plays a functional role in this extrahypothalamic brain region.

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