+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Trh in the rat cerebellum 2. uptake by cerebellar slices

, : Trh in the rat cerebellum 2. uptake by cerebellar slices. Peptides (Orlando) 2(3): 283-288

The in vitro uptake of exogenous TRH by rat cerebellar slices was investigated. TRH was taken up via a process sharing many of the properties of a high affinity transport system, i.e., saturation kinetics; high affinity kinetic constants (Km1 = 1.06 .times. 10-5 M, Km2 = 5.6 .times. 10-6 M); temperature sensitivity (Q10 = 1.48); dependency on the Na concentration in the incubation medium; and tissue/medium ratios greater than 1. After 60 min of incubation at C in the presence of bacitracin, the tissue/medium ratio was 5:1. Under these conditions 70% of the total label was recovered as [3H-Pro]TRH. These findings demonstrated the existence of removal mechanisms for TRH in the cerebellum and gave further support to the hypothesis that TRH plays a functional role in this extrahypothalamic brain region.

Accession: 006839737

PMID: 6795609

Submit PDF Full Text: Here

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Pacheco, M.F.; Woodward, D.J.; McKelvy, J.F.; Griffin, W.S., 1981: Trh in the rat cerebellum: II. Uptake by cerebellar slices. We investigated the in vitro uptake of exogenous TRH by rat cerebellar slices. TRH was found to be taken up via a process sharing many of the properties of a high affinity transport system, viz: (1) saturation kinetics; (2) high affinity kinetic c...

Kimura H., 1988: The inhibitory and excitatory neurotransmitters in the cerebellum intradendritic study in the guinea pig cerebellar slices. Japanese Journal of Pharmacology 46(SUPPL): 44P

Larsell O.; Jansen J.; Korneliussen H.K.; Mugnaini E., 1972: The comparative anatomy and histology of the cerebellum the human cerebellum cerebellar connections and cerebellar cortex. The Comparative Anatomy And Histology Of The Cerebellum The Human Cerebellum Cerebellar Connections And Cerebellar Cortex 268

Rafaelsen, O.J., 1961: Action of insulin on glucose uptake of rat brain slices and isolated rat cerebellum. Glucose uptake of "second" brain slices was 10 to 15% greater than that of "first" brain slices from rats. Insulin (10-1 i.[mu]./ml) increased the glucose uptake of first brain slices by 10 to 15%, whereas it had no effect on t...

Takahashi, M.; Sarantis, M.; Attwell, D., 1998: Glutamate uptake in Purkinje cells in rat cerebellar slices. We have described how whole-cell clamping of neurons in brain slices has allowed a characterization of postsynaptic transporters, probably a mixture of EAAC1 and EAAT4, in cerebellar Purkinje cells. Similar experiments have been carried out on tra...

Collins, J.M.; Seeds, N.W., 1983: Binding and uptake of putative neurotransmitters in mutant mouse cerebellum and cerebellar reaggregat. γ-Amino butyric acid (GABA) appears to be the inhibitory transmitter of various cerebellar interneurons, while glutamate has been suggested as the excitatory transmitter of granule cells. In this study we have determined the developmental profile...

Beas-Zárate, C.; Morales-Villagran, A.; Tapia-Arizmendi, G.; Feria-Velasco, A., 1991: Effect of 3-acetylpyridine on serotonin uptake and release from rat cerebellar slices. The cerebellum receives indolaminergic fibers influencing Purkinje cell discharges. Data from our laboratories have demonstrated an endogenous release of serotonin (5-HT) and a Na(+)-dependent uptake and Ca(2+)-dependent release of [3H]5-HT from s...

Takahashi, M.; Attwell, D., 1995: Electrogenic glutamate uptake in Purkinje cells in rat cerebellar slices. Journal of Physiology (Cambridge) 483P(0): 62P

de Barry, J.; Vincendon, G.; Gombos, G., 1983: Uptake and metabolism of L-[3H]glutamate and L-[3H]glutamine in adult rat cerebellar slices. Using very low concentrations (1 mumol range) of L-2-3-[3H]glutamate, (3H-Glu) or L-2-3-[3H]glutamine (3H-Gln), we have previously shown by autoradiography that these amino acids were preferentially taken up in the molecular layer of the cerebella...

Lehmler, H.-Joachim; Robertson, L., W.; Garrison, A.W.yne; Kodavanti, P.-Rao, S., 2005: Effects of PCB 84 enantiomers on -phorbol ester binding in rat cerebellar granule cells and Ca-45(2+)-uptake in rat cerebellum. There is evidence that polychlorinated biphenyl (PCB) congeners with ortho chlorine substituents have potential to cause neurotoxicity. Many PCB congeners implicated in these neurotoxic effects are chiral. It is currently unknown if the enantiomer...