EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Trh mobilizes calcium ion from endoplasmic reticulum and mitochondria of gh 3 pituitary cells characterization of cellular calcium ii pools by a method based on digitonin permeabilization


, : Trh mobilizes calcium ion from endoplasmic reticulum and mitochondria of gh 3 pituitary cells characterization of cellular calcium ii pools by a method based on digitonin permeabilization. Proceedings of the National Academy of Sciences of the United States of America 79(20): 6294-6298

Treatment of 45Ca2+-loaded [rat] GH3 pituitary [tumor] cells with various concentrations of digitonin revealed discrete pools (I and II) of cellular 45Ca2+ defined by differing detergent sensitivities. Markers for cytosol and intracellular organelles indicated that the 2 45Ca2+ pools were correlated with the 2 major cellular Ca2+-sequestering organelles, endoplasmic reticulum (I) and mitochondria (II). Studies with various inhibitors were consistent with these assignments. Mitochondrial uncouplers preferentially depleted 45Ca2+ pool II while trifluoperazine selectively depleted 45Ca2+ pool I. Control experiments indicated that translocation of in situ organellar 45Ca2+ during and after permeabilization was negligible. The digitonin-permeabilization method was used to examine the effect of TRH treatment on intracellular Ca2+-pools of GH3 pituitary cells. TRH rapidly depleted both endoplasmic reticulum and mitochondrial exchangeable Ca2+ by 25-30%. The 45Ca2+ loss from both pools was maximal by 1 min after TRH addition and was followed by a recovery phase; mitochondrial 45Ca2+ content returned to control levels by 30 min. Previous treatment of cells with the mitochondrial uncoupler carbonyl cyanide p-trifluoromethoxy-phenylhydrazone blocked TRH-induced 45Ca2+ efflux from mitochondria, while previous treatment with valinomycin, an agent that depleted both 45Ca2+ pools, blocked any additional effect of TRH on these pools, TRH rapidly promotes a net loss of exchangeable Ca2+ from GH3 cells as a result of hormone-induced mobilization of Ca2+ from endoplasmic reticulum and mitochondria.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 006839773

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Shahnaz Dawar; Younus, S. M.; Zaki, M. J., 2007: Use of Eucalyptus sp., in the control of Meloidogyne javanica root knot nematode. Different parts of Eucalyptus sp., viz., leaves, stem, bark and fruit used as aqueous and ethanol extracts showed nematicidal effect against Meloidogyne javanica root knot nematode, reduced hatching of eggs, increased mortality of juveniles with...

Schneider, R. E.; Viteri, F. E., 1972: Morphological aspects of the duodenojejunal mucosa in protein-calorie malnourished children and during recovery. Stereoscopical and light microscopy studies of biopsy samples from the duodenojejunal mucosa, D-xylose absorption tests, Schilling tests and faeces fat balances were made in 4 healthy and 11 malnourished Guatemalan children.Study by microscopy sho...

Swinnard, L.; Garcia, M.; Weis, D.; Ito, G.; Flinders, A.; Bianco, T.; Appelgate, B.; Taylor, B.; Blay, C.; Yamasaki, S.; Tagami, T., 2008: Geochemical, geological and geophysical inferences for the origin of the South Kauai Swell. Geochimica et Cosmochimica Acta 72.12S: A922

Martins Neto Marcelo A., 1998: The Espinhaco Supergroup in Minas Gerais; the record of a Paleo-Mesoproterozoic rift-sag basin. The aim of this work is to present a model for the tectono-sedimentary evolution of the Espinhaco Basin in Minas Gerais. The model is supported by integrated sedimentological, paleogeographical, stratigraphical, structural and tectonic studies, ca...

Baldwin Barrett; Sheaffer Yvonne, 1970: Faint meteor ablation processes. Journal of Geophysical Research 75(2): 495-498

Falcon Lang Howard J.; Calder John H., 2004: UNESCO world heritage and the Joggins Cliffs of Nova Scotia. UNESCO World Heritage status is the highest honour that may be bestowed on a palaeontological site. In addition to heightening conservation status, it confers international recognition of a locality's "outstanding universal value" a...

Blight, G., 2009: Solar heating of the soil and evaporation from a soil surface. In 1994 a system for classifying landfills for municipal solid waste by local climate was introduced in South Africa. If it can be shown that potential evaporation from the landfill surface will exceed average annual rainfall, it is accepted that...

Strachan P.J.; Wilson J.S.; Kelly N.C., 1987: Response of dairy cows to compounds varying in level and profile of undegradable essential amino acids. Animal Production 44(3): 492-493

Szabo Gyula; Guczi Judit; Koerdel Werner; Zsolnay Adam; Major Veronika; Keresztes Peter, 1999: Comparison of different HPLC stationary phases for determination of soil-water distribution coefficient, K (sub oc) , values of organic chemicals in RP-HPLC system. Chemosphere (Oxford) 39(3): 431-442

Anonymous., 1992: Erich Geist. Natur und Museum Frankfurt am Main 122(6): 200