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Trh rapidly enhances phosphorus 32 labeled ortho phosphate incorporation into phosphatidic acid in cloned gh 3 cells


Biochemical & Biophysical Research Communications 101(1): 124-130
Trh rapidly enhances phosphorus 32 labeled ortho phosphate incorporation into phosphatidic acid in cloned gh 3 cells
TRH enhanced incorporation of [32P]orthophosphate into phospholipids in GH3 cells, cloned rat pituitary [tumor] cells that produce prolactin. [32P]Orthophosphate incorporation into phosphatidic acid was significantly increased 30 s after TRH addition and attained a steady-state level after 2 min. [32P]Orthophosphate incorporation into phosphatidylinositol was not significantly increased until 2 min after TRH. [32P]Orthophosphate incorporation into phosphatidylcholine and phosphatidylethanolamine were not affected. TRH had no effect on [32P]orthophosphate uptake by GH3 cells for at least 2 min, but stimulated 45Ca2+ efflux from GH3 cells within 15 s. Since TRH enhancement of [32P]orthophosphate incorporation into phosphatidic acid was temporally associated with its effect on 45Ca2+ efflux and Ca2+ appears to be a critical intracellular regulator of prolactin release, acidic phospholipid metabolism may be involved in TRH-induced, Ca2+-mediated prolactin secretion.

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Accession: 006839781



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