EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Trh receptor binding sites autoradiographic distribution in the rat and guinea pig brain


Journal of Neurochemistry 45(5): 1448-1463
Trh receptor binding sites autoradiographic distribution in the rat and guinea pig brain
Thyrotropin-releasing hormone (TRH) binding sites were labeled in vitro in mounted brain tissue sections from rat and guinea pig brains with [3H]methyl TRH and localized autoradiographically using 3H-sensitive film. Regional densities of TRH binding sites were measured by computer-assisted microdensitometry. The distribution of sites in both species was highly heterogeneous. In both guinea pig and rat brains, the highest densities of binding sites were seen in the amygdaloid nuclei and the perirhinal cortex. In contrast, in other brain areas, a clear difference between the distribution of sites in rat and guinea pig was found. The temporal cortex, pontine nuclei, and interpeduncular nucleus, which contained high densities of binding in the guinea pig, were scarcely labeled in the rat. The accessory olfactory bulb and the septohippocampal area presented in the rat higher concentrations of binding sites than in the guinea pig. Other brain areas showing intermediate to low densities in both species were accumbens nucleus, bed nucleus of the stria terminalis, dentate gyrus, facial and hypoglossal nuclei, and gelatinosus subnucleus of the trigeminal nerve, among others. The anterior pituitary also presented low to intermediate concentrations of receptors. The distribution of TRH sites here described does not completely correlate with that of endogenous TRH, but is in good agreement with previous biochemical data. The results are discussed in correlation to the physiological effects that appear to be mediated by TRH.

(PDF 0-2 workdays service: $29.90)

Accession: 006839786



Related references

Thyrotropin-releasing hormone receptor binding sites: autoradiographic distribution in the rat and guinea pig brain. Journal of Neurochemistry 45(5): 1448-1463, 1985

Distribution and characterization of 5 ht 1d binding sites in the guinea pig brain an autoradiographic analysis. European Journal of Pharmacology 183(3): 1109-1110, 1990

Autoradiographic distribution of tritiated dextro pentazocine binding sites in guinea pig brain. Van Ree, J M , Et Al (Ed ) International Congress Series, No 914 New Leads in Opioid Research; International Narcotics Research Conference, Noordwijkerhout, Netherlands, July 8-13, Xxvii+391p Elsevier Science Publishers B V (Biomedical Division): Amsterdam, Netherlands; (Dist in The Usa And Canada By Elsevier Science Publishing Co , Inc : New York, New York) Illus 263-265, 1990

An autoradiographic comparison of NK3 receptor binding sites in guinea-pig brain using - neurokinin B and Senktide. British Journal of Pharmacology 116(PROC SUPPL ): 253P, 1995

Autoradiographic distribution of [3H](+)-pentazocine and [3H]1,3-di-o-tolylguanidine (DTG) binding sites in guinea pig brain: a comparative study. Brain Research 581(1): 33-38, 1992

Comparative pharmacological properties and autoradiographic distribution of [3H]ethylketocyclazocine binding sites in rat and guinea pig brain. Life Sciences 33 Suppl 1: 183-186, 1983

Autoradiographic distribution of tritium labelled dextro pentazocine binding sites in guinea pig brain. Society for Neuroscience Abstracts 16(1): 801, 1990

3H]sumatriptan labels both 5-HT1D and 5-HT1F receptor binding sites in the guinea pig brain: an autoradiographic study. Naunyn-Schmiedeberg's Archives of Pharmacology 352(3): 263-275, 1995

Autoradiographic distribution and receptor binding profile of [125I]Bolton Hunter-rat amylin binding sites in the rat brain. Journal of Pharmacology and Experimental Therapeutics 270(2): 779-787, 1994

Autoradiographic Distribution and Receptor Binding Profile of (125I)Bolton Hunter-Rat Amylin Binding Sites in the Rat Brain. Journal of Pharmacology and Experimental Therapeutics 270(2): 779-787, 1994