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Trh receptors in regulation of prolactin gene expression in gh cells


Biochemical & Biophysical Research Communications 106(3): 811-817
Trh receptors in regulation of prolactin gene expression in gh cells
Three GH cell strains (rat pituitary tumor cells in culture) which synthesize different amounts of prolactin (PRL) and which exhibit a differential response to TRH, have been utilized to study the TRH receptor-modulated regulation of PRL gene expression. Equilibrium [3H]TRH binding studies revealed that, PRL-producing (PRL+) GH4C1 and 928-9b GH substrains possess TRH specific receptors with similar affinities for the ligand. The total number of TRH receptors per cells in TRH-nonresponsive 928-9b strain is 6.5 times < that which could be detected in TRH-responsive GH4C1 cells. Epidermal growth factor (EGF) also stimulates PRL synthesis in GH4C1 cells. Unlike TRH, EGF stimulates PRL synthesis in TRH-nonresponsive 928-9b cells, suggesting that nuclear events necessary for the stimulation of PRL gene expression in 928-9b cells are operative. 5 Bromodeoxyuridine (BrdUrd) induces PRL synthesis in PRL-nonproducing (PRL-) F1BGH12C1 cells. PRL induction in BrdUd-treated cells can not be further stimulated by TRH or EGF.


Accession: 006839789



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