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Trh receptors in regulation of prolactin gene expression in gh cells

, : Trh receptors in regulation of prolactin gene expression in gh cells. Biochemical & Biophysical Research Communications 106(3): 811-817

Three GH cell strains (rat pituitary tumor cells in culture) which synthesize different amounts of prolactin (PRL) and which exhibit a differential response to TRH, have been utilized to study the TRH receptor-modulated regulation of PRL gene expression. Equilibrium [3H]TRH binding studies revealed that, PRL-producing (PRL+) GH4C1 and 928-9b GH substrains possess TRH specific receptors with similar affinities for the ligand. The total number of TRH receptors per cells in TRH-nonresponsive 928-9b strain is 6.5 times < that which could be detected in TRH-responsive GH4C1 cells. Epidermal growth factor (EGF) also stimulates PRL synthesis in GH4C1 cells. Unlike TRH, EGF stimulates PRL synthesis in TRH-nonresponsive 928-9b cells, suggesting that nuclear events necessary for the stimulation of PRL gene expression in 928-9b cells are operative. 5 Bromodeoxyuridine (BrdUrd) induces PRL synthesis in PRL-nonproducing (PRL-) F1BGH12C1 cells. PRL induction in BrdUd-treated cells can not be further stimulated by TRH or EGF.

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Related references

Mu, M.C.; Chakrabarti, S.; Hanes, S.D.; Biswas, D.K., 1982: Thyrotropin releasing hormone receptors in regulation of prolactin gene expression in GH cells. Biochemical and Biophysical Research Communications 106(3): 811-817

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