+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Tri acyl glycerol synthesis in isolated fat cells evidence that the sn glycerol 3 phosphate acyl transferase ec and di hydroxy acetone phosphate acyl transferase ec activities are dual catalytic functions of a single microsomal enzyme

, : Tri acyl glycerol synthesis in isolated fat cells evidence that the sn glycerol 3 phosphate acyl transferase ec and di hydroxy acetone phosphate acyl transferase ec activities are dual catalytic functions of a single microsomal enzyme. Journal of Biological Chemistry 251(18): 5738-5744

The acyl-CoA[Coenzyme A]:-sn-glycerol-3-phosphate acyltransferase (EC (glycerol-P acyltransferase) and acyl-CoA:dihydroxyacetone phosphate acyltransferase (EC (DHAP acyltransferase) activities were investigated in vitro in order to evaluate the quantitative contribution of the glycerol-P and DHAP pathways for the synthesis of triacylglycerols in isolated fat cells [rat] and to test the hypothesis that these 2 activities may be dual catalytic functions of a single enzyme. More than 85% of both acyltransferase activities was associated with the microsomal subcellular fraction. The microsomal glycerol-P acyltransference activity showed an apparent Km of 8 .mu.M for glycerol-P with a Vmax of 15.6 nmol/min per mg, while the DHAP acyltransferase activity showed an apparent Km of 40 .mu.M for DHAP with a Vmax of 9.7 nmol/min per mg. Glycerol-P was a competitive inhibitor (Ki = 7.2 .mu.M) of the DHAP acyltransferase, and DHAP was a competitive inhibitor (Ki = 92 .mu.M) of the glycerol-P acyltransferase. The 2 acyltransferase activities showed virtual identity in their pH dependence, acyl-CoA chain length dependence, thermolability and inactivation by N-ethylmaleimide. Trypsin, detergents, collagenase, phospholipases and various salts and organic solvents also had similar effects on both activities. The microsomal glycerol-P and DHAP acyltransferase activities probably actually represent dual functions of a single enzyme. Calculations based on the above kinetic constants and previously reported glycerol-P and DHAP pools in adipocytes suggest that the in vivo ratio of glycerol-P to DHAP acylation should be greater than 24:1.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006839854

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Suzuki, H.; Shirato, T.; Kanyama, T.; Nakagawa, H.; Yazaki, S., 1975: Study of mucle relaxants with evoked electromyography. 6. Fluctuation of the M2/M1 ratio by repetitive paired stimulation. Masui. Japanese Journal of Anesthesiology 24(10): 980-987

Morin, L.; Auld, B.A.; Brown, J.F.; Cholil, M.A.dul, 1994: Pathogenic fungi occurring on the Noogoora Burr complex in Australia. The Noogoora burr (cocklebur) complex in Australia consists of four closely related Xanthium species considered to be among the most important weeds in summer crops and pasture. In 1987, a mail survey was sent to agronomists to determine the distr...

Bennett, G.G.; Steinberg, D.M.; Stoute, C.; Lanpher, M.; Lane, I.; Askew, S.; Foley, P.B.; Baskin, M.L., 2015: Electronic health (eHealth) interventions for weight management among racial/ethnic minority adults: a systematic review. Electronic health (eHealth) interventions have demonstrated efficacy for weight management. However, little is known about their efficacy among racial/ethnic minority populations, in whom there is a disproportionate prevalence of obesity. This sys...

Hanke, T.; Takizawa, H.; Busch, D.; Pamer, E.; Altman, J.; Raulet, D.H., 1998: The MHC class I specificities of inhibitory Ly49 molecules. Natural Immunity 16(2-3): 56, Feb

Anonymous, 2009: Cholera: global surveillance summary, 2008. Releve Epidemiologique Hebdomadaire 84(31): 309-324

Gulanick, M.; Keough, V., 1997: Focus groups: an exciting approach to clinical nursing research. The focus group interview is an exciting qualitative research design that is gaining popularity among nurse clinicians and researchers. This article provides a practical approach to designing and implementing focus groups. The authors' own re...

Demarco de Hormaeche, R.; Thornley, M.J.; Glauert, A.M., 1978: Demonstration by light and electron microscopy of capsules on gonococci recently grown in vivo. A study by light microscopy, using Leishman's stain alone or Leishman's stain followed by nigrosin, showed the presence of capsules on gonococci of two strains subcultured from subcutaneous chambers in guinea pigs. With the Alcian blue m...

MOORE, R.A., 1962: The problem of abstinence by the patient as a requisite for the psychotherapy of alcoholism. I. The need for abstinence by the alcoholic patient during treatment. Quarterly Journal of Studies on Alcohol 23: 105-111

Gansky, S.A.; Ellison, J.A.; Rudy, D.; Bergert, N.; Letendre, M.A.; Nelson, L.; Kavanagh, C.; Walsh, M.M., 2005: Cluster-Randomized Controlled Trial of An Athletic Trainer-Directed Spit (Smokeless) Tobacco Intervention for Collegiate Baseball Athletes: Results After 1 Year. Context: Athletes in the United States are at high risk for using spit (smokeless) tobacco (ST) and incurring its associated adverse health effects.Objective: To examine whether an athletic trainer-directed ST intervention could decrease initiatio...

Gross, S.J.; Shime, J.; Farine, D., 1987: Shoulder dystocia: predictors and outcome. A five-year review. Shoulder dystocia is an uncommon complication of delivery with a high morbidity rate. Ninety-one cases were coded for shoulder dystocia at the Toronto General Hospital from 1980 through 1985. True shoulder dystocia was found in 24 cases, an incide...