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Tri carboxylic acid cycle activity in relation to itaconic acid biosynthesis by aspergillus terreus


, : Tri carboxylic acid cycle activity in relation to itaconic acid biosynthesis by aspergillus terreus. Journal of General Microbiology 129(9): 2877-2884

Cell-free extracts of an itaconic acid producing strain of A. terreus contained NADP-dependent isocitrate dehydrogenase activity but no NAD-dependent activity was detected. During the acid-producing phase the activity of the NADP-linked enzyme fell to 1/8 of its value during the growth phase, whereas citrate synthase activity increased. Intact mitochondria were isolated from growth and production phase mycelium. The preparation from the growth phase oxidized exogenous NADH and all tricarboxylic acid cycle intermediates tested. That from the production phase oxidized tricarboxylic acid cycle intermediates at a reduced rate but exogenous NADH oxidation remained high. [2-14C]Acetate was incorporated into itaconic acid and over 90% of the incorporated radioactivity was located in the methylene carbon. [1-14C]-Acetate was a poorer precursor and did not preferentially label the methylene C. These results support the view that itaconic acid biosynthesis in A. terreus occurs by decarboxylation of aconitic acid, the latter produced by the normal reactions of the tricarboxylic acid cycle.

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Bernier, J. L., 1942: Bacteriologic and immunologic studies in dental caries A preliminary report. Exposed surfaces of extracted teeth with extensive carious lesions containing a large amount of leathery decay were sterilized with 95% phenol. All superficial debris was carefully removed, the bulk of the carious dentin removed en masse, placed i...

Waheed M.A.; Basu P.K., 1970: The effect of air pollutants on the eye part 1 the effect of an organic extract on the conjunctival goblet cells. Canadian Journal of Ophthalmology 5(3): 226-230

Klinger, N.; Partanen, J., 1999: Microsatellite markers and MHC haplotype variation in Finland. Human Immunology 60(SUPPL 1): S108

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Schlemmer, Scott R.; Sirotnak, Francis M., 1995: Facile Preparation of Inside-Out Plasma Membrane Vesicles from Tumor Cells for Functional Studies of Pharmacologically Relevant Translocating ATPases. A reasonably facile and effective procedure is described for the preparation of inside-out plasma membrane vesicles from tumor cells. The method incorporates nitrogen cavitation, optimized with respect to the applied N-2 pressure, in the absence o...

Mikulski P.; Wiglusz R.; Delag G.; Galuszko E.; Mikulska T., 1976: Toxicological evaluation of typical fiber glass reinforced poly ester plastic applied in shipbuilding. Bulletin of the Institute of Maritime & Tropical Medicine in Gdynia 27(2): 167-175

Wytock, G. P., 1993: The effect of sowing date and weed competition on the growth and yield of oilseed rape in Scotland. In field trials conducted in Aberdeenshire, Scotland, during 1989-1991, the effects were evaluated of sowing dates (2 dates 9-14 d apart) and timing of weed control (hand weeding, or metazachlor at 750 g at growth stages (GS) 1.00-1.01 followed by...

Trojan, J.; Stuermer, M.; Teuber, G.; Berger, A.; Faust, D., 2007: Treatment of patients with lamivudine-resistant and adefovir dipivoxil-resistant chronic hepatitis B virus infection: is tenofovir the answer?. Gut 56(3): 436-437

Finch, L. R., 1967: Further studies of amino acid activation during the life cycle of the blowfly Lucilia cuprina. A technique has been developed for preparing from the soluble fraction of Lucilia cuprina an enzyme system containing about 80% of the RNA and 25% of the total ATP-pyrophosphate exchange activity of the soluble fraction. Sixteen different amino ac...

Nakamura, M.; Takeda, K.; Yang, Q.; Hisamatsu, T.; Hanai, N.; Nakamura, Y.; Morimoto, S.; Mori, I.; Kakudo, K., 2003: Osteoclast-like cells express receptor activity modifying protein 2 Application of laser capture microdissection. Modern Pathology 16(1): 325A, January