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Tri dimensional reconstruction of the mitochondria of the ginkgo in tissue culture study using thick and serial thin sections


, : Tri dimensional reconstruction of the mitochondria of the ginkgo in tissue culture study using thick and serial thin sections. Canadian Journal of Botany 57(4): 332-340

The observation of thick sections and serial thin sections with the electron microscope demonstrates a reticular organization of mitochondria in cultured cells from Ginkgo. Thick sections show that most of the mitochondria fuse either in loose networks made up of elongated elements or in dense clusters composed of globular units. Serial thin sections help to prove the true reticular nature of the mitochondrial organization; most of the mitochondrial profiles are part of highly fused and branched networks. These mitochondrial reticula (about 2-3/cell) occupy an important place within the cell. Some mitochondria remain isolated or they fuse slightly. Two 3-dimensional models were built: the 1st one constructed with cardboard sheets is used to diaplay the organization of a reticulum; the other of Plexiglas clearly indicates the relative positions and the form of mitochondria in a complete cell. The mitochondria, plastids and vacuoles of this cell are also measured and enumerated.

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