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Tri iodo thyronine stimulates specifically growth hormone messenger rna in rat pituitary tumor cells


, : Tri iodo thyronine stimulates specifically growth hormone messenger rna in rat pituitary tumor cells. Proceedings of the National Academy of Sciences of the United States of America 74(5): 2054-2058

In a cell free protein synthesizing system from a rabbit reticulocyte lysate, total RNA extracted from cultured rat pituitary tumor (GH3) cells directed, in a dose related manner, the synthesis of proteins that were precipitated by antisera specific to rat growth hormone (somatotropin) and rat prolactin. A marked decrease in growth hormone secretion and growth hormone mRNA activity was observed when cells were grown in a medium deficient in thyroid hormone. Addition of triiodothyronine in physiologic amounts prevented and completely reversed this effect within 48 h. Thyroid hormone had no effect on prolactin secretion or prolactin mRNA activity. These data suggest that thyroid hormone may stimulate synthesis of growth hormone through induction of transcriptional activity. The possibility of an additional effect at the posttranscriptional level was not excluded. Although thyroid hormone is believed to have a general effect on a variety of metabolic processes, some effects, at the molecular level, may be quite selective, as indicated by the observed changes in growth hormone but not prolactin mRNA activity. The GH3 cell model is useful in the study of triiodothyronine action because of independence from secondary hormonal effects caused by hypothyroidism and because simultaneous measurement of prolactin mRNA activity serves as a unique internal control.

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Kumara-Siri, M.H.; Surks, M.I., 1985: Regulation of growth hormone messenger rna synthesis by 3 5 3' triiodo l thyronine in cultured growth hormone producing rat pituitary tumor cells gc cells dissociation between nuclear iodothyronine receptor concentration and growth hormone messenger rna synthesis during the dna synthesis phase of the cell cycle. 3,5,3'-Triiodo-L-thyronine (T3) regulates the growth rate and GH production of cultured GC cells, a rat piutuitary tumour cell line. We have previously demonstrated a parallel increase in cellular content of DNA and nuclear T3 glucocorticoid...

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Seo, H.; Vassart, G.; Brocas, H.; Refetoff, S., 1977: Triiodothyronine stimulates specifically growth hormone mRNA in rat pituitary tumor cells. In a cell-free protein-synthesizing system from a rabbit reticulocyte lysate, total RNA extracted from cultured rat pituitary tumor (GH3) cells directed, in a dose-related manner, the synthesis of proteins that were precipitated by antisera specif...

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