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Triplet minus singlet absorbance difference spectra of reaction centers and antenna pigments of the green photosynthetic bacterium prosthecochloris aestuarii



Triplet minus singlet absorbance difference spectra of reaction centers and antenna pigments of the green photosynthetic bacterium prosthecochloris aestuarii



Biochimica et Biophysica Acta 767(2): 200-208



Absorbance-detected ESR in zero magnetic field was applied to several pigment-protein complexes that belong to the membrane-bound photosystem of the green sulfur bacterium P. aestuarii. Three triplet states can be discerned that are formed in the light-harvesting bacteriochlorophyll (BChl) a protein, the core complex and the primary donor P-840, respectively. Triplet-minus-singlet absorbance difference spectra of the latter 2 states are presented. The spectrum of the core complex shows a bleaching at 837 nm and an absorbance increase at 808 nm. This suggests a strong electronic interaction between at least 2 of the constituent BChl a molecules of the complex. The triplet-minus-singlet spectrum of P-840 shows 2 negative bands at 826 and 837 nm, that, according to their linear dichroism, have almost parallel polarization. No spectral evidence exists for the presence of 2 resolved dimer exciton bands of P-840. Thus, P-840 either consists of 2 weakly coupled BChl a molecules or of a strongly coupled pair with 1 allowed exciton band at 837 nm, the other blue-shifted exciton component being very weak. Decay rates of PT-840 of 6790 (.+-. 500) s-1, 3920 (.+-. 300) s-1 and 1275 (.+-. 100) s-1 were observed for the x, y and z triplet sublevels, respectively.

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Accession: 006842241

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DOI: 10.1016/0005-2728(84)90188-9


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