Tumor-specific immunity induced by somatic hybrids. I. Lack of relationship between immunogenicity and tumorigenicity of selected hybrids
Kim, B.S.; Liang, W.; Cohen, E.P.
Journal of Immunology 123(2): 733-738
Hybrid clones were derived from fusion of TEPC-15 plasmacytoma cells of BALB/c mice with mouse L [neoplastic fibroblast] cells of C3H origin. The moprhology, tumorigenicity and immunogenicity of 3 representative clones were extensively studied. One clone (LTC-1) showed a morphology intermediate to that of either parental cell and possessed the highest tumorigenic and immunogenic properties. The other 2 clones displayed a flat morphology which differed significantly from that of either parent. One of these 2, LTC-4, eventually induced tumors in some (BALB/c .times. C3H)F1 mice but failed to stimulate protective immunity against TEPC-15 tumor cells in BALB/c mice. The other hybrid clone, LTC-2, has a very flat morphology and did not induce tumors, although it was capable of stimulating a significant level of tumor immunity. Histologically, all the tumors induced by hybrid cells were firbosarcomas rather than plasmacytomas. The morphology of hybrid cells may be correlated with the tumorigenicity and the histologic appearance of tumor. The degree of tumorigenicity of individual hybrid clones does not correspond to their immunogenicity in the host. Major antigens responsible for immunogenicity may not play an important role in induction of tumors.