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Ultra cytochemical localization of alkaline phosphatase ec 3.1.3.1 isozymes in human cancer cell lines in vitro



Ultra cytochemical localization of alkaline phosphatase ec 3.1.3.1 isozymes in human cancer cell lines in vitro



Acta Histochemica et Cytochemica 14(3): 223-235



Biochemically identified cancer-associated alkaline phosphatase (orthophosphoric monoester phosphohydrolase, EC 3.1.3.1) isozymes were studied by EM cytochemistry in 4 human cancer cell lines, as chorion isozyme in KMK-2, Regan isozyme in CaR-1 and HeLa S3-10, and Kasahara isozyme in HeLa S3-5. Using ultracytochemical techniques, the chorion isozyme in KMK-2 cells was demonstrated almost exclusively on the microvilli facing the culture medium and not on the lateral surface adjacent to the neighboring cells with interdigitating folds. In both HeLa sublines, thought to represent more immature cells, the Regan and Kasahara isozymes were demonstrated on the whole plasma membrane. The Regan isozyme in CaR-1 cells was strongly demonstrated in the intracytoplasmic microcysts in addition to the whole plasma membrane. These localizations might be related to the differentiation and deviated formation of the plasma membrane in cancer cells.

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