EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Ultrastructural demonstration of oxytocin and vasopressin release sites in the neural lobe and median eminence of the rat by tannic acid and immunogold methods






Neuroscience Letters 74(2): 151-157

Ultrastructural demonstration of oxytocin and vasopressin release sites in the neural lobe and median eminence of the rat by tannic acid and immunogold methods

We have investigated the localization of oxytocin (OXT) and vasopressin (AVP) release sites in the neural lobe and median eminence (ME) of the rat. Ultrastructural and post-embedding immunocytochemical studies with protein A-gold as a marker were used for the identification of OXT and AVP neurosecretory granules (NSG). Release of the contents of these OXT and AVP NSG by exocytosis has been clearly demonstrated with the tannic acid-Ringer incubation method in axon terminals of the neural lobe and in varicose fibers with OXT and AVP NSG in the internal zone of the ME. As these fibers lack synaptic specializations, and are not located in the direct vicinity of capillaries of the primary portal plexus, these observations suggest that OXT and AVP are released in a paracrine way in the ME of the rat.

Accession: 006857925

PMID: 3574755

DOI: 10.1016/0304-3940(87)90141-8

Download PDF Full Text: Ultrastructural demonstration of oxytocin and vasopressin release sites in the neural lobe and median eminence of the rat by tannic acid and immunogold methods



Related references

Ultrastructural immuno cytochemical localization of neurophysin and vasopressin in the neural lobe and median eminence of guinea pig. Anatomical Record: 479-480, 1975

Effect of catecholamines and histamine on vasopressin release from the median eminence or the neural lobe in vitro. Federation Proceedings 38(3 PART 1): 1026, 1979

Effect of salt loading and salt deprivation on the vasopressin and oxytocin content of the median eminence and the neural lobe in adrenalectomized rats. Cell and Tissue Research 246(2): 413-423, 1986

In vitro release of vasopressin and oxytocin from rat median eminence tissue. Neuroendocrinology 42(6): 481-488, 1986

Ultrastructural characterization of exocytotic release sites with combination of tannic acid and immuno gold methods. Ultramicroscopy 21(2): 186, 1987

Ultrastructural characterization of exocytotic release sites in different layers of the median eminence of the rat. Cell and Tissue Research 252(1): 107-114, 1988

A new technique for post-embedding immunogold localization of vasopressin in the rat median eminence. Acta Histochemica 95(1): 75-78, 1993

Accumulation and release of vasotocin and mesotocin from the frog Rana ridibunda median eminence and neural lobe in respons to de- and rehydration. Annales d'Endocrinologie, 421: 69-70, 1981

Stimulatory effects of Cd2+ and Co2+ on release of vasopressin and oxytocin from isolated rat neural lobe. FASEB Journal 17(4-5): Abstract No 324 7, March, 2003

Involvement of eicosanoids in release of oxytocin and vasopressin from the neural lobe of the rat pituitary. Endocrinology 116(6): 2663-2668, 1985