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Ultrastructural modifications of seeds of allium cepa hydrated or not hydrated and subjected to low temperatures minus 196 celsius



Ultrastructural modifications of seeds of allium cepa hydrated or not hydrated and subjected to low temperatures minus 196 celsius



Canadian Journal of Botany 58(22): 2367-2379



Osmic fixation was used in an ultrastructural study of A. cepa seeds, both in the quiescent state and at the beginning of imbibition, in order to complete earlier observations made after glutaraldehyde fixation. In embryonic radicles, various groups of weakly stained lamellar structures looked like endoplasmic reticulum profiles. In these quiescent tissues, osmic fixation revealed many vesicles which appeared to be atypical elements of endoplasmic reticulum. In the first few hours of imbibition, reactive structures were more numerous and more diverse. Cisternae and vesicles of endoplasmic reticulum became typical looking, such as classically described in active tissues of other species. When A. cepa seeds were treated with liquid N (-196.degree. C), induced structural alterations depended on the initial state of hydration. Lamellar systems underwent morphological changes. Osmic fixation showed the presence of numerous opaque globules throughout the cytoplasm. These globules seemed to derive from cisternae that appeared to have become fragmented during freezing.

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Accession: 006859261

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