Ultrastructural peroxidase cytochemistry of 3 established human myelogenous leukemia cell lines hl 60 kg 1 and ml 2

Himori, T.; Tanaka, T.; Ohnuma, T.

Leukemia Research 9(7): 913-920


ISSN/ISBN: 0145-2126
Accession: 006859790

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The ultrastructural peroxidase cytochemistry of 3 established human acute myelogenous leukemia cell lines (HL-60, KG-1 and ML-2) was studied to clarify the cytochemical differences and the differences in maturation/differentiation stages. HL-60 cells contained azurophil granules which exhibited strong peroxidase reaction. The more central region of individual granules is less reactive than the outer portion of these granules. The secretory apparatus of HL-60 cells, i.e, rough endoplasmic reticulum, perinuclear cisterna and Golgi apparatus, also contained peroxidase-reactive material. ML-2 cells contained azurophil granules with peroxidase reaction; nuclear membranes were peroxidase-negative and thin cisternae of rough endoplasmic reticulum exhibited only a faint peroxidase reaction. KG-1 cells were peroxidase-negative. KG-1 is classified as the least mature of the cell lines studied. ML-2 cells are considered early promyelocytes and HL-60 cells as late promyelocytes.