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Uptake of cadmium by a cadmium chloride tolerant bacterium pseudomonas aeruginosa

Uptake of cadmium by a cadmium chloride tolerant bacterium pseudomonas aeruginosa

Journal of Fermentation Technology 57(4): 273-279

Uptake of Cd by a cadmium chloride-tolerant bacterium, P. aeruginosa G-1, already isolated from activated sludge by the authors, was investigated. Observation with a scanning electron microscope of cells growing with Cd showed the absence of sticky materials adhering to cells which were usually seen in the absence of Cd2+. The Cd-tolerant bacterium tolerated Cd2+ and Cu2+, but not Cr6+ and Hg2+. On chemical analysis, about 88% of Cd taken up by the cells was found in the cytoplasm, and about 12% in the cell envelope fraction. Cd uptake into the cytoplasm fraction indicated that Cd was dispersed in both protein and nucleic acid fractions. The Cd distribution into the insoluble fraction showed that most of the Cd was found in the polyphosphate-polysaccharide fraction.

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Accession: 006870378

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