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Use of a clonal assay for cyto toxic t lymphocytes to determine the ly phenotypes of the cyto toxic precursor and effector cells to allo antigens and tri nitro phenyl modified self antigens



Use of a clonal assay for cyto toxic t lymphocytes to determine the ly phenotypes of the cyto toxic precursor and effector cells to allo antigens and tri nitro phenyl modified self antigens



Journal of Immunology 125(5): 1977-1986



Considerable controversy has arisen regarding the Ly phenotypes of cytotoxic T lymphocytes (CTL) and their precursors (CLP) to alloantigens and modified-self antigens. Although there is general agreement that all CTL and their pregenitors express the Ly-2 alloantigen, the presence of the Ly-1 alloantigen on CTL or CLP is debated. Clonal assays for CLP, capable of detecting single CLP in the absence of accessory cells, have been developed. This assay system provides a sensitive means of determining the Ly phenotypes of CLP to alloantigens or trinitrophenyl- (TNP) modified self antigens. Lymph node cells from C57BL/6 (Ly1.2, 2.2, 3.2) or CBA (Ly-1.1, 2.1, 3.2) mice were treated with anti-Ly serum and complement (C); the frequencies of CLP of the treated populations to alloantigens or TNP-modified self antigens were determined. The number of CLP reactive to alloantigens or TNP-modified self antigens were greatly reduced after treatment with anti-Ly-1 or anti-Ly-2 serum and C in C57BL/6 and CBA mice. The CLP to alloantigens or TNP-modified self antigens in these 2 strains of mice apparently are Ly 1+2+. The CTL derived from the Ly1+2+ CLP were also Ly1+2+. The significance of this finding with respect to the cytotoxic repertoire for alloantigens and modified self antigens is discussed.

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Accession: 006875572

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