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Use of biospecific adsorbents with poly saccharide spacers in affinity chromatography

Klyashchitsky, B.A.; Mitina, V.Kh; Morozevich, G.E.; Yakubovskaya, R.I.

Journal of Chromatography 210(1): 67-76

1981

ISSN/ISBN: 0021-9673
Accession: 006877038
The isolation of mouse liver poly(A)-mRNA and purification of trypsin and [human serum] kallikrein using new biospecific adsorbents having dextran, glycogen or amylopectin spacers are described as the first examples of affinity chromatography on such adsorbents. A comparative study of these adsorbents and similar adsorbents without spacers was carried out. The optimum conditions for affinity chromatography of poly(A)-mRNA were considered.

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Related References

Klyashchitsky, B.A.; Mitina, V.Kh 1981: Affinity adsorbents with poly saccharide spacers preparation and properties Journal of Chromatography 210(1): 55-66
Klyashchitskii, B.A.; Koroleva, G.E.; Mitina, V.Kh; Alekhina, R.P.; Zborovskaya, I.B.; Likhtenshtein, A.V. 1979: Isolation and purification of bio polymers by affinity chromatography part 1 new highly effective biospecific adsorbents for bio affinity chromatography of poly adenylated messenger rna Bioorganicheskaya Khimiya 5(1): 92-99
Klyashchitskii, B.A.; Koroleva, G.E.; Mitina, V.Kh; Alekhina, R.P.; Zborovskaya, I.V.; Likhtenshtein, A.V. 1979: Isolation and purification of bio polymers by affinity chromatography 1. new highly effective biospecific adsorbents for the affinity chromatography of poly adenylic acid containing messenger rna Soviet Journal of Bioorganic Chemistry 5(1): 70-77
Mitina, V.K. ; Frantsuzova, N.A.; Kutsenko, N.G.; Zolotov, N.N.; Klyashchitskii, B.A. 1985: Isolation and purification of biopolymers by biospecific affinity chromatography 8. comparative studies of biospecific adsorbents based on sepharose and spheron for affinity chromatography of fibronectin Bioorganicheskaya Khimiya 11(2): 173-179
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Alvarez, I.C.I.; Strumia, M.C.; Bertorello, H.E. 1997: Preparation of adsorbents applicable to pseudobiospecific ligand affinity chromatography using different spacers and ligands International Conference on Applied Physical Chemistry 34(1): 103-111
Winzor, D.J.; Yon, R.J. 1984: Biospecific elution and multivalency in affinity chromatography: reappraisal of the biospecific desorption of rat liver lactate dehydrogenase from 10-carboxydecylamino-Sepharose Biochemical Journal 217(3): 867-868
Safarik, I. 1991: Magnetic biospecific affinity adsorbents for lysozyme isolation Biotechnology Techniques 5(2): 111-114
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Hutchens, T.W.; Yip, T.T. 2004: Retentate chromatography--profiling with biospecific interaction adsorbents Official Gazette of the United States Patent and Trademark Office Patents 1288(1)