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Use of the perifusion technique on rat thyroid fragments in the study of thyroid hormone secretion short term effects of thyrotropin theophylline and glucagon



Use of the perifusion technique on rat thyroid fragments in the study of thyroid hormone secretion short term effects of thyrotropin theophylline and glucagon



Journal of Endocrinology 102(1): 43-48



Perifusion of rat thyroid fragments was performed to study short-term effects of TSH, theophylline and glucagon on thyroid hormone secretion. This technique proved to be relatively convenient and sensitive and gave reproducible results for at least 3 h, permitting precise kinetic studies of response to hormonal and pharmacological agents without any interference. There was a significant (P < 0.001) linear correlation between the log TSH concentrations over the range 20-150 mU/ml and thyroid response. A 2nd stimulation, using the same concentration of TSH, did not differ from the 1st stimulation if they were separated by an active washing period of only 15 min. Theophylline also had a stimulating effect and like TSH induced an early release of the hormone fraction with a peak between 2 and 4 min, but it did not potentiate the TSH effect. Perifusion of rat thyroid fragments was a useful tool for analyzing dynamic effects of various substances. These effects were significant for periods of time as short as 20 min. Each thyroid preparation could be used a 2nd time for another pharmacological or hormonal test. Apparently there was a direct glucagon effect on thyroid hormone secretion with a dose-response correlation.

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Accession: 006882630

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PMID: 6736852



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