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Uv radiation induced neoplastic transformation of normal human cells in vitro



Uv radiation induced neoplastic transformation of normal human cells in vitro



Chemico-Biological Interactions 36(1): 45-60



Human foreskin cell cultures in scheduled DNA synthesis (S phase) of the cell cycle were exposed to UV irradiation at a dose of 10 J .cntdot. m-2 in the presence of insulin. Treated cell populations were selectively passaged in a high amino acid supplemented complete growth medium (CM) after 20 Dulbecco's phosphate buffered saline (pH 6.8) (PDL) and were grown in soft agar. Treated cell populations were grown in 1% serum-supplemented growth medium and at 41.degree. C in 10% serum supplemented growth medium. Cell populations 4-5 PDL after treatment exhibited altered colony morphology and altered lectin agglutination profiles and would not grow in soft agar, events probably associated with the early stages in the expression of the transformed phenotype. After 20 PDL the cells would grow in soft agar at a frequency of 20 colonies/105 cells seeded in soft agar. The cell populations derived from these colonies, when propagated and injected into nude mice, formed myxofibromas at the injection sites rather than forming fibrosarcomas, the type of tumor previously described for chemical carcinogen-induced neoplasms.

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