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Uv radiation induced production of rhizobium meliloti mutants with changed symbiotic properties



Uv radiation induced production of rhizobium meliloti mutants with changed symbiotic properties



Sel'skokhozyaistvennaya Biologiya (9): 44-49



Exposure of R. meliloti strain CXM1 gave 23 mutants for symbiotic properties. Among them are: those without nitrogen fixation (3), without root nodule formation on alfalfa (5), with cluster-shaped nodule formation (2) and also with a decreased or increased nitrogen fixing activity of efficiency (13). It is shown that mutants for nitrogen fixing activity, efficiency and nodule morphology frequently arise from low doses (60 Dj/m2), whereas Nod- and Fix- mutants predominantly arise from high doses (240 Dj/m2). A significant correlation was established between the indices of symbiotic activity of mutants in microvegetational and pot experiments (r = 0.75-0.88), and consequently microvegetational experiments may be recommended for use in initial mass evaluation of strains for practical breeding.

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