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Variable chlorophyll a fluorescence from p 700 enriched photosystem i particles dependent on the redox state of the reaction center

Variable chlorophyll a fluorescence from p 700 enriched photosystem i particles dependent on the redox state of the reaction center

Biochimica et Biophysica Acta 504(1): 153-164

Photosystem I particles enriched in P-700 prepared by Triton X-100 treatment of [spinach] chloroplasts show a light-induced increase in fluorescence yield of more than 100% in the presence of dithionite but not in its absence. Steady state light maintains the P-700 of these particles in the oxidized state when ascorbate is present, but in the presence of dithionite only a transient oxidation occurs. EPR data show that, in these particles, the primary electron acceptor (X) is maintained in the reduced state by light at room temperature only when the dithionite is also present. The secondary electron acceptors are reduced in the dark by dithionite. Fluorescence emission and excitation spectra and fluorescence lifetime measurements for the constant and variable fluorescence indicate a heterogeneity of the chlorophyll in these particles. Apparently the variable fluorescence comes from those chlorophylls which can transfer their energy to the reaction center and the states PX and P+X are more effective quenchers of chlorophyll fluorescence than PX-, where P is P-700.

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Accession: 006891496

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PMID: 213109

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