Yeast phenylalanyl transfer rna synthetase evidence for the formation of adp by phosphorolysis of enzyme bound aminoacyladenylate
Guedon, G.; Ebel J P.; Remy, P.
Biochimie 69(11-12): 1175-1182
ADP and Ap3A are synthesized by the yeast phenylalanyl-tRNA synthetase, according to reaction pathways similar to the pyrophosphorolysis of the intermediate aminoacyladenylate or to the one leading to Ap4A synthesis. The enzyme-bound phenylalanyladenylate reacts with inorganic phophate or ADP to yield, respectively, ADP or Ap3A. The rate of synthesis is strongly stimulated by Zn2+. This new phosphorolysis activity accounts for the complex pattern of bisnucleoside polyphosphate syntheses starting from ATP.