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Chapter 6,930

3 cyano 4 methylumbelliferyl beta d galactopyranoside a new chromogenic and fluorigenic substrate for the beta galactosidase from escherichia coli

Markaryan, A.N.; Voznyi, Y.V.

Bioorganicheskaya Khimiya 16(4): 569-570

1990

ISSN/ISBN: 0360-4497
Accession: 006929036
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A novel substrate, 3-cyano-4-trifluoromethylumbelliferyl-.beta.-D-galactopyranoside, for the continuous (pKa = 6,0 for the measured product), photometric (.epsilon. = 42,000 M-1 cm-1 at 440 nm, pH 7,5) and fluorimetric (.lambda.ex = 440 nm, .lambda.em = 500 nm) assay of .beta.-galactosidase was synthesized. The substrates affinity to nitrocellulose membranes made possible its use for sensitive (up to 0.1 mole of the enzyme in 5-10 min) visual identification of the .beta.-galactosidase activity on nitrocellulose sheets.

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Related References

Yegorov, A.M.; Markaryan, A.N.; Vozniy, Y.V.; Cherednikova, T.V.; Demcheva, M.V.; Berezin, I.V. 1988: 4 trifluoromethylumbelliferyl beta d galactopyranoside its synthesis and application as the fluorigenic substrate of beta galactosidase escherichia coli for screening monoclonal antibodies by immunosorbent elisa Analytical Letters 21(2): 193-210
Tanaka, K.; Ishikawa, E. 1986: A highly sensitive bioluminescent assay of beta d galactosidase from escherichia coli using 2 nitrophenyl beta d galactopyranoside as a substrate Analytical Letters 19(3-4): 433-444
Bainbridge, B.W.; Mathias, N.; Price, R.G.; Richardson, A.C.; Sandhu, J.; Smith, B.V. 1991: Improved methods for the detection of beta galactosidase activity in colonies of escherichia coli using a new chromogenic substrate vbztm gal 2 2 4 beta d galactopyranosyloxy 3 methoxyphenylvinyl 3 methylbenzothiazolium toluene 4 sulfonate FEMS Microbiology Letters 80(2-3): 319-324
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