3 cyano 4 methylumbelliferyl beta d galactopyranoside a new chromogenic and fluorigenic substrate for the beta galactosidase from escherichia coli
Markaryan, A.N.; Voznyi, Y.V.
Bioorganicheskaya Khimiya 16(4): 569-570
ISSN/ISBN: 0360-4497 Accession: 006929036
A novel substrate, 3-cyano-4-trifluoromethylumbelliferyl-.beta.-D-galactopyranoside, for the continuous (pKa = 6,0 for the measured product), photometric (.epsilon. = 42,000 M-1 cm-1 at 440 nm, pH 7,5) and fluorimetric (.lambda.ex = 440 nm, .lambda.em = 500 nm) assay of .beta.-galactosidase was synthesized. The substrates affinity to nitrocellulose membranes made possible its use for sensitive (up to 0.1 mole of the enzyme in 5-10 min) visual identification of the .beta.-galactosidase activity on nitrocellulose sheets.