+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A domain at the 3' end of the polymerase gene is essential for encapsidation of coronavirus defective interfering RNAs



A domain at the 3' end of the polymerase gene is essential for encapsidation of coronavirus defective interfering RNAs



Journal of Virology 65(6): 3219-3226



Two murine hepatitis virus strain A59 defective interfering (DI) RNAs were generated by undiluted virus passages. The DI RNAs were encapsidated efficiently. The smallest DI particle, DI-a, contained a 5.5-kb RNA consisting of the following three noncontiguous regions from the MHV-A59 genome, which were joined in frame: the 5'-terminal 3.9 kb, a 798-nucleotide fragment from the 3' end of the polymerase gene, and the 3'-terminal 805 nucleotides. A full-length cDNA clone of the DI-a genome was constructed and cloned downstream of the bacteriophage T7 promoter. Transcripts derived from this clone, pMIDI, were used for transfection of MHV-A59-infected cells and found to be amplified and packaged. Deletion analysis of pMIDI allowed us to identify a 650-nucleotide region derived from the 3' end of the second open reading frame of the polymerase gene that was required for efficient encapsidation.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 006941381

Download citation: RISBibTeXText

PMID: 2033672


Related references

Translation but not the encoded sequence is essential for the efficient propagation of the defective interfering RNAs of the coronavirus mouse hepatitis virus. Journal of Virology 69(6): 3744-3751, 1995

Structure and encapsidation of transmissible gastroenteritis coronavirus (TGEV) defective interfering genomes. Advances in Experimental Medicine and Biology 380: 583-589, 1995

Defective-interfering particles of murine coronavirus: mechanism of synthesis of defective viral RNAs. Virology 163(1): 104-111, 1988

Defective interfering influenza RNAs of polymerase 3 gene contain single as well as multiple internal deletions. Virology 124(2): 232-237, 1983

Assembled coronavirus from complementation of two defective interfering RNAs. Journal of Virology 71(5): 3922-3931, 1997

Complete sequence analyses show that two defective interfering influenza viral RNAs contain a single internal deletion of a polymerase gene. Proceedings of the National Academy of Sciences of the United States of America 79(7): 2216-2220, 1982

Replication of synthetic defective interfering RNAs derived from coronavirus mouse hepatitis virus-A59. Virology 216(1): 174-183, 1996

Analysis of efficiently packaged defective interfering RNAs of murine coronavirus: localization of a possible RNA-packaging signal. Journal of Virology 64(12): 6045-6053, 1990

Analysis of cis-acting sequences essential for coronavirus defective interfering RNA replication. Virology 197(1): 53-63, 1993

Structural requirements in an essential segment of tomato bushy stunt virus defective interfering RNAS. 1995

Conserved motifs in a tombusvirus polymerase modulate genome replication, subgenomic transcription, and amplification of defective interfering RNAs. Journal of Virology 89(6): 3236-3246, 2015

A single amino acid mutation in the PA subunit of the influenza virus RNA polymerase promotes the generation of defective interfering RNAs. Journal of Virology 77(8): 5017-5020, 2003

Effect of small interfering RNAs on in vitro replication and gene expression of feline coronavirus. American Journal of Veterinary Research 75(9): 828-834, 2014

Genome nucleotide lengths that are divisible by six are not essential but enhance replication of defective interfering RNAs of the paramyxovirus simian virus 5. Virology 232(1): 145-157, 1997