+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

A second locus for the 5S multigene family in Secale L.: sequence divergence in two lineages of the family

A second locus for the 5S multigene family in Secale L.: sequence divergence in two lineages of the family

Genome 32(3): 457-467

ISSN/ISBN: 0831-2796

PMID: 2545523

DOI: 10.1139/g89-469

The 5S RNA genes in Secale sp. are arranged as tandem arrays of a 460- and 480-bp repeating sequence. These size classes were initially discovered by restriction endonuclease analysis using BamHI and subsequently by DNA sequencing of cloned units. The length variation between short and long units originated from major deletion-insertion events in the noncoding spacer region of the 5S DNA repeat units. In situ hybridization with [3H]cRNA and biotin-labelled probes synthesized from both the short and long 5S DNA units of S. cereale localized the sites on chromosome 1R and a new site on a chromosome identified as 5R. We propose that the chromosome 1R locus, which has been mapped previously, be named 5SDna-R1 and the second locus, reported in the present paper, be referred to as 5SDna-R2. A preferential hybridization of a probe from the long unit to the 5SDna-R2 locus and of a probe from the short unit to the 5SDna-R1 locus is reported. The clustering of long units in the 5SDna-R2 locus was confirmed by restriction endonuclease digestion of DNA from rye chromosome 5R additions to wheat. Nucleotide sequence alignment of 5S DNA repeat units from a number of Secale species, using both phenetic and cladistic computer programmes, demonstrated that two clear lineages corresponding to the long and short units existed in this genus. The different Secale species could not be unambiguously differentiated using the 5S DNA sequences.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 006965112

Download citation: RISBibTeXText

Related references

A second locus for the 5S multigene family in Secale L.: sequence divergence in two lineages of the family. Genome (Ottawa. Print) 32(3): 456-467, 1989

Multigene family isoform profiling from blood cell lineages. Bmc Genomics 3(1): 22, 2002

Intermediate filament proteins a multigene family distinguishing major cell lineages. Trends In Biochemical Sciences: 469-472, 1986

Nucleotide variation and divergence in the histone multigene family in Drosophila melanogaster. Genetics 122(1): 87-97, 1989

Three members of the S multigene family are linked to the S locus of Brassica. Molecular and General Genetics: Mgg 256(3): 257-264, 1997

Application of Whole Genome Sequencing and Pan-Family Multi-Locus Sequence Analysis to Characterize Relationships Within the Family Brucellaceae. Frontiers in Microbiology 11: 1329, 2020

The MGEA6 multigene family has an active locus on 14q and at least nine pseudogenes on different chromosomes. Genomics 75(1-3): 43-48, 2001

Subfamily divergence in the multigene family of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcS) in Triticeae and its relatives. Genes and Genetic Systems 73(5): 297-309, 1998

Structural divergence is more extensive than sequence divergence for a family of intrinsically disordered proteins. Proteins 81(10): 1686-1698, 2013

Evolutionary divergence among lineages of the ocean sunfish family, Molidae (Tetraodontiformes). Marine Biology (Berlin) 148(2): 405-414, 2005

Locus-specific cDNA cloning in the class I multigene family: structure of H-2Dr and H-2Ds. Immunogenetics 32(6): 456-459, 1990

Physical mapping of the 5S rRNA multigene family in 6x triticale and rye: identification of a new rye locus. Genome 38(3): 623-626, 1995

Expression of the S-locus receptor kinase multigene family in Brassica oleracea. Plant Journal: for Cell and Molecular Biology 6(6): 807-813, 1994

Episodic evolution and rapid divergence of members of the rat multigene family encoding the salivary prohormone-like protein SMR1. Molecular Biology and Evolution 13(6): 758-766, 1996

Physical mapping of the 18S.26S rRNA multigene family in common wheat: identification of a new locus. Chromosoma 100(2): 71-78, 1991