Section 7
Chapter 6,971

A study on the sequence of phototoxic effects of rose bengal using retinal pigment epithelial cells in vitro

Menon, I.A.; Basu, P.K.; Persad, S.D.; Rosatone, S.; Wiltshire, J.D.

Experimental Eye Research 49(1): 67-73


ISSN/ISBN: 0014-4835
PMID: 2759192
DOI: 10.1016/0014-4835(89)90076-6
Accession: 006970940

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This paper describes a study on the sequence of the phototoxic effects of rose bengal (RB), a fluorescein derivative used as a vital stain in the diagnosis of certain external ocular diseases. Bovine melanotic RPE cells were grown in culture. These cells were labeled with [51Cr] and exposed of visible light in the presence of various concentrations of RB;the leakage of [51Cr] from the cells was used as a measure of cell lysis. Exposure to light of the cells with 0.3-10 .mu.M RB induced approximately 13 to 43% cell lysis. The lysis progressively increased when the exposure time was varied from 10 to 30 min. A relatively short period of irradiation in the presence of RB was sufficient to produce sublytic cellular injury which could subsequently lead to complete cell lysis even in the absence of the photochemical treatment. The dark reaction was time-dependent, and reached a maximum for a given irradiation period. Our results thus show that there are two different processes that could eventually lead to the cell lysis: (a) a phototoxic effect which caused a sublytic damage and (b) a dark reaction that followed.

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