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Adaptations of Goldner's Masson trichrome stain for the study of undecalcified plastic embedded bone

Adaptations of Goldner's Masson trichrome stain for the study of undecalcified plastic embedded bone

Biotechnic & Histochemistry 67(1): 30-34

Specialized adaptations for application of Goldner's Masson trichrome stain to plastic embedded undecalcified bone specimens are presented. This stain can be used successfully on methyl-glycol methacrylate, glycol methacrylate and Spurr embedded bones. The stain affords the advantage of good cellular staining due to the hematoxylin component with concomitant sharp discrimination of mature bone matrix which stains green, immature new bone matrix which stains red, and calcified cartilage which stains very pale green. Use of red filters during photomicrography aids in bone-osteoid discrimination in black and white photographs.

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Accession: 006987269

Download citation: RISBibTeXText

PMID: 1617000

DOI: 10.3109/10520299209110002

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