+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Adhesion of frog pronephric tumor cells to normal cells cultivated on microcarrier beads



Adhesion of frog pronephric tumor cells to normal cells cultivated on microcarrier beads



Clinical & Experimental Metastasis 8(3): 227-240



The processes of cell adhesion and active spreading were assessed between frog normal pronephric, pronephric tumor and heterologous liver cells. Confluent monolayers were developed on collagen-coated microcarrier beads, then exposed to homologous or heterologous cells and cultivated with a rotary (orbital) flask culture technique at 23.degree. C. All three cell lines attach and actively spread on the collagen-coated microcarrier beads. Secondary attachment of normal (non-transformed) proliferating cells to their confluent monolayers occurs but cell spreading is restrained. Dissociated pronephric tumor cells adhere and actively spread on the surfaces of normal pronephric cells, and eventually encapsulate them. Normal pronephric cells do not adhere readily to the cell surfaces of pronephric tumor cells grown on microcarrier beads. Tumor cells also attach, actively spread and overgrow heterologous liver cells attached to microcarrier beads. Suboptimal temperatures (17.degree. C) slow tumor cell attachment and spreading on normal cells. Colder temperature (8.degree. C) permits tumor cell attachment and adhesion to normal cell-coated beads but active cell spreading is prohibited. The same temperture retards cell spreading directly on the collagen-coated beads.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 006988982

Download citation: RISBibTeXText

PMID: 2183959

DOI: 10.1007/bf00141254


Related references

Intraocular bioassay of a pronephric tumor cell line cultivated on macroporous microcarrier beads. In Vitro Cellular and Developmental Biology. Animal 30a(2): 85-86, 1994

Intraocular Bioassay of a Pronephric Tumor Cell Line Cultivated on Macroporous Microcarrier Beads. In Vitro Cellular & Developmental Biology. Animal 30A(2): 85-86, 1994

Normal and hybrid frog gastrula cells show differences in cell adhesion to fibronectin sepharose beads. Anatomical Record: 61a-62a, 1986

Increased humoral and cellular immunity in mice inoculated with allogeneic tumor cells attached to microcarrier beads. Immunology Letters 6(1): 39-43, 1983

Effects of agitation rate on aggregation during beads-to-beads subcultivation of microcarrier culture of human mesenchymal stem cells. Cytotechnology 69(3): 503-509, 2016

The use of microcarrier beads in ion transport NMR studies of perfused cells. Journal of Magnetic Resonance. Series B 108(1): 81-85, 1995

Culture of endocrine cells from the rat fetal pancreas on microcarrier beads. Diabetes Research & Clinical Practice 5(SUPPL 1): S332, 1988

Interleukin 1: production by P388D1 cells attached to microcarrier beads. Journal of Immunological Methods 46(2): 197-204, 1981

Normal frog gastrula extracellular materials serve as a substratum for normal and hybrid cell adhesion when covalently coupled with CNBr-activated Sepharose beads. Cell Differentiation 10(1): 47-55, 1981

Normal frog gastrula extracellular materials serve as a substratum for normal and hybrid cell adhesion when covalently coupled with cyanogen bromide activated sepharose beads. Cell Differentiation 10(1): 47-56, 1981

Characterization of a perfusion reactor utilizing mammalian cells on microcarrier beads. Biotechnology Progress 16(3): 471-479, 2000

The use of cytodex microcarrier beads in patch-clamp studies on cultured epithelial cells. Pflugers Archiv 413(1): 90-92, 1988

Flow-induced modulation of the permeability of endothelial cells cultured on microcarrier beads. Journal of Cellular Physiology 168(2): 403-411, 1996

Proliferation of rat Pneumocystis carinii on cells sheeted on microcarrier beads in spinner flasks. Journal of Clinical Microbiology 31(6): 1659-1662, 1993

Adult rat pancreatic islet cells adherent to microcarrier beads: evaluation of function and morphology. In Vitro Cellular and Developmental Biology 21(9): 485-487, 1985