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Agroinfection of wheat a comparison of agrobacterium strains


Plant Science (Shannon) 63(2): 247-256
Agroinfection of wheat a comparison of agrobacterium strains
Fifteen strains of Agrobacterium were invested for their ability to agroinfect wheat dwarf virus into 4-day-old wheat seedlings. Viral infection was determined by ELISA. The greatest proportion of plants infected by any strain was 55%, achieved by the Agrobacterium rhizogenes strain Ar2629. In general A. rhizogenes strains were found to give a higher proportion of agroinfected plants than A. tumefaciens strains. Nopaline types of A. tumefaciens were superior to octopine types in their ability to transfer DNA to wheat plants, but some strains of the latter type did infect to a low frequency. Seedling size at inoculation had no effect on the number of plants that developed a viral infection, although seedlings with shoots less than 1.0 cm long were not likely to survive damage caused by the inoculation procedure. The experiment was replicated over time and no significant variation was found between the three sets of data obtained.


Accession: 006995203

DOI: 10.1016/0168-9452(89)90250-1



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