Section 8
Chapter 7,006

An ammonium dependent protein synthesis cell free system for halobacteria

Sanz, J.L.; Marin, I.; Balboa, M.A.; Urena, D.; Amils, R.

Biochemistry 27(21): 8194-8199


ISSN/ISBN: 0006-2960
Accession: 007005820

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An efficient poly(U)-dependent polyphenylalanine system for extreme halophilic archaebacteria is described. The system was tested with eight different species including the six genera described to date: Halobacterium, Haloferax, Haloarcula, Halococcus, Natronobacterium, and Natronococcus. In all of them the optimal ionic conditions are similar. The system is extremely dependent on NH4+, with the optimal concentration being 1.5 M (NH4)2SO4, and has virtually no dependence on K+. The internal concentration of NH4+ and K+ of three halobacteria with different degrees of halophilia was measured. A close correlation between the internal concentration of K+ and the external concentration of cations, as well as a relatively low concentration of NH4+, was obtained in all cases. The possible importance of all the variables of the system is discussed.

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