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An improved preservation method of chicken semen in liquid nitrogen



An improved preservation method of chicken semen in liquid nitrogen



Research Bulletin of the Aichi-ken Agricultural Research Center (21): 351-355



Many practices of the Lake's freezing method for preservation of chicken semen had been done in Japan, but resulted in low fertility. We made some modifications on the method and the key points were as follows: (1) In order to protect sperm from cold shock, semen collected from White Leghorn males was immediately diluted in a glycerol-free Lake's colution. (2) The Lake's glycerolised solution was added stepwise to this diluted semen at an interval of 1 min in the following procedure; times of 0.1ml, one time of 0.2ml, 3 times of 0.4ml and one time of 0.7ml per 1ml of diluted semen. (3) The re-diluted semen was transferred to a freezing apparatus without any temperature rising, and was cooled by 5.degree. C/min down to -30.degree. C. Three times artificial insemination with the frozen-thawed semen brought about 90% fertility rate during the first week.

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Accession: 007011242

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