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Analysis of DNA from a Beta procumbens chromosome fragment in sugar beet carrying a gene for nematode resistance



Analysis of DNA from a Beta procumbens chromosome fragment in sugar beet carrying a gene for nematode resistance



Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 79(5): 663-672



We have begun to apply techniques for the preparation and anaylsis of large DNA segments from sugar beet (Beta vulgaris) addition lines carrying a mitotically stable chromosome fragment from B. procumbens that confers monogenic resistance to the nematode Heterodera schachtii, with a view towards isolating the resistance gene. DNA probes specific for this chromosome fragment were selected, and various methods for cloning genome-specific fragments, including probes from megabase DNA separated in pulsed-field slab gels, are compared. Probes that display high homology to B. procumbens have been used for hybridization of a representative genomic library and for initial step in mapping the chromosome fragment via pulsed-field gel electrophoresis after restriction with infrequently cutting enzymes. Our data indicate that DNA molecules from the entire chomosome fragment can be separated from protoplast DNA lysates.

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Accession: 007015628

Download citation: RISBibTeXText

PMID: 24226582

DOI: 10.1007/bf00226881


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