+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Analysis of lactulose i. automated enzymatic micromethod for the determination of lactulose



Analysis of lactulose i. automated enzymatic micromethod for the determination of lactulose



Milchwissenschaft 46(9): 555-558



An efficient automated micromethod for the enzymatic determination of lactulose is described. Disadvantages of the official macromethod, e.g. time expenditure and high consumption of enzymes, are eliminated. By miniaturizing the complete working process, the consumption of reagents and enzymes could be reduced by a factor of 10. 48 samples could be analyzed in one run with low work and time expenditure. In comparison to the chromatographic methods suggested by the International Dairy Federation, the method described combines a high sample throughput with excellent detection sensitivity and reproducibility.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 007016350

Download citation: RISBibTeXText


Related references

Continuous synthesis of lactulose in an enzymatic membrane reactor reduces lactulose secondary hydrolysis. Bioresource Technology 167: 108-115, 2014

Automated enzymatic assays for the determination of intestinal permeability probes in urine. 1. Lactulose and lactose. Clinica Chimica Acta; International Journal of Clinical Chemistry 187(2): 79-87, 1990

Enzymatic production of lactulose and 1-lactulose: current state and perspectives. Applied Microbiology and Biotechnology 97(14): 6167-6180, 2013

Expression and characterization of two β-galactosidases from Klebsiella pneumoniae 285 in Escherichia coli and their application in the enzymatic synthesis of lactulose and 1-lactulose. Zeitschrift für Naturforschung. C Journal of Biosciences 69(11-12): 479-487, 2015

Effects of purified lactulose powder (P-511), lactulose powder and lactulose syrup on the blood ammonia concentration and the pH of intestinal contents in intact rats and Eck's fistula dogs. Oyo Yakuri 54(3): 175-182, 1997

Automated determination of lactulose in milk using an enzyme reactor and flow analysis with integrated dialysis. Analytica Chimica Acta. 324(1): 37-45, 1996

Determination of lactulose in lactulose syrup. Ceskoslovenska Farmacie 23(2): 61-64, 1974

Enzymatic determination of lactulose. Zeitschrift fuer Lebensmittel-Untersuchung und -Forschung 171(6): 443-445, 1980

Milk. Determination of lactulose content. Enzymatic method. International IDF Standard ( 175: 1995): 4 pp., 1995

A new enzymatic spectrophotometric assay for the determination of lactulose in milk. Analytica Chimica Acta 406(2): 217-224, 2000

Influence of chronic lactulose ingestion on the colonic metabolism of lactulose in man (an in vivo study). Journal of Clinical Investigation 75(2): 608-613, 1985

Improved reproducibility of the lactulose/rhamnose absorption test using a 1 vs 10 gram lactulose dose. Gastroenterology 114(4 Part 2): A427, 1998

Production of 1-lactulose and lactulose using commercial β-galactosidase from Kluyveromyces lactis in the presence of fructose. Food Chemistry 137(1-4): 1-7, 2013

Transformation of lactulose trihydrate into anhydrous lactulose by fluidized bed drying and its characterization. Carbohydrate Research 339(6): 1069-1075, 2004

An improved colorimetric method for the estimation of lactulose in lactose-lactulose mixtures. Food chemistry3(5): 399-402, 1992