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Analysis of lens cell fate and eye morphogenesis in transgenic mice ablated for cells of the lens lineage

Analysis of lens cell fate and eye morphogenesis in transgenic mice ablated for cells of the lens lineage

Development 106(3): 457-463

ISSN/ISBN: 0950-1991

PMID: 2598819

Transgenic mice carrying the diphtheria toxin A gene driven by mouse .gamma.2-crystallin promoter sequences manifest microphthalmia due to ablation of fiber cells in the ocular lens. Here we map ablation events in the lens by crossing animals hemizygous for the ablation construct with transgenic mice homozygous for the in situ lacZ reporter gene driven by identical .gamma.2-crystallin promoter sequences. By comparing the spatial distribution of lacZ-expressing cells and the profile of .gamma.-crystallin gene expression in the lenses of normal and microphthalmic offspring, the contributins of specific cell types to lens development were examined. The results suggest that phenotypically and developmentally distinct populations of lens fiber cells are able to contribute to the lens nucleus during organogenesis. We also show that dosage of the transgene and its site of integration influence the extent of ablation. In those mice homozygous for the transgene and completely lacking cells of the lens lineage, we show that the sclera, cornea, and ciliary epithelium are reduced in size but, otherwise, reasonably well formed. In contrast, the anterior chamber, iris, and vitreous body are not discernible while the sensory retina is highly convoluted and extensively fills the vitreous chamber.

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Accession: 007016376

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