Section 8
Chapter 7,054

Benzyladenine and light stimulated plastid protein synthesis in excised cucumber cotyledons

Ohya, T.; Suzuki, H.

Plant Physiology and Biochemistry 28(1): 27-36


ISSN/ISBN: 0981-9428
Accession: 007053621

Etiolated excised cucumber cotyledons (Cucumis sativus) were treated with benzyladenine (BA) in darkness for 18 h and then exposed to light. This treatment markedly enhanced the in organello protein synthesis activity of etioplasts, though the direct addition of BA to the isolated plastids had no effect. Light stimulated the synthesis of a 32 kDa and several low-molecular polypeptides of thylakoid membranes and the large subunit of ribulose bisphosphate carboxylase (EC, which were all barely detectable in etioplasts from unilluminated cotyledons. In darkness BA stimulated the synthesis of a 39 kDa and several membrane polypeptides lower than 34 kDa and the large subunit of ribulose bisphosphate carboxylase. However, no significant change in the level of mRNA for the carboxylase large subunit could be observed upon the pretreatment with BA in darkness.

PDF emailed within 1 workday: $29.90